BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I13028 1 BBa_I13028 cI (TP-901) QPI intermediate 2004-07-14T11:00:00Z 2015-08-31T04:07:32Z Released HQ 2013 -- No description -- false false _6_ 0 159 7 In stock false false jenmitch component939472 1 BBa_C0075 component939462 1 BBa_B0034 annotation939472 1 BBa_C0075 range939472 1 19 597 annotation939462 1 BBa_B0034 range939462 1 1 12 BBa_C0075 1 cI TP901 TP901 cI repressor from phage TP901-1 (+LVA) 2004-01-27T12:00:00Z 2015-08-31T04:07:23Z TP901-1 Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false Dimeric repressor from bacteriophage TP901-1 with dissociation constant of 2*10^-9.<br/><br/> Sequence was translated with the E.Coli K12 codon usage table, and nucleotides 84-86 was changed from TTC to TTT to avoid the cut site GAATTC (81-86).<br/><br/> Bronsted, L., Hammer, K., Johansen, A.H., 2003. Identification of operator sites of the CI repressor of the phage TP901-1: evolutionary link to the other phages. Virology 311, 144-156. <P>cI sequence slightly different from part C0051. NCBI locus: NC_001416 true crackdots annotation306574 1 LVA range306574 1 541 573 annotation302645 1 cI tp901 range302645 1 1 540 annotation2214007 1 Help:Barcodes range2214007 1 580 604 BBa_B0034_sequence 1 aaagaggagaaa BBa_C0075_sequence 1 atgaaaaccgataccagtaacagattaaaacaaataatggcggaacgcaacctgaaacaggtggatatcttaaacttatcgattccatttcagaaaaaatttggtatcaagctgagtaaaagtacgctgtcgcagtatgtgaactctgtgcagtcgccagaccagaaccgcatctatttactggcgaaaacgctgggcgtttccgaagcttggctgatgggatttgatgttccaatggttgaatcgtcgaaaatcgaaaacgattcggaaaacatcgaagaaaccattacggtgatgaaaaaattggaagagccgagacagaaagtggtcctggataccgccaaaattcagctgaaagaacaggatgagcagaacaaagtgaaacagatcgaagattaccgtctaagtgatgaatatttagaagaacagatcagtaaagcgagtgcatatggaggtggccaattaaatgacaacgataaagaatttttcaaacgtttgttgaaaaacacgttaaaagaaaaaattgataaaggagatctggctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_I13028_sequence 1 aaagaggagaaatactagatgaaaaccgataccagtaacagattaaaacaaataatggcggaacgcaacctgaaacaggtggatatcttaaacttatcgattccatttcagaaaaaatttggtatcaagctgagtaaaagtacgctgtcgcagtatgtgaactctgtgcagtcgccagaccagaaccgcatctatttactggcgaaaacgctgggcgtttccgaagcttggctgatgggatttgatgttccaatggttgaatcgtcgaaaatcgaaaacgattcggaaaacatcgaagaaaccattacggtgatgaaaaaattggaagagccgagacagaaagtggtcctggataccgccaaaattcagctgaaagaacaggatgagcagaacaaagtgaaacagatcgaagattaccgtctaagtgatgaatatttagaagaacagatcagtaaagcgagtgcatatggaggtggccaattaaatgacaacgataaagaatttttcaaacgtttgttgaaaaacacgttaaaagaaaaaattgataaaggagatctggctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z