LOCUS BBa_I13035 3003 bp DNA linear 22-Nov-2024 DEFINITION 3OC6HSL Receiver Device with Inducible Control of LuxR and a YFP Output device ACCESSION BBa_I13035 VERSION BBa_I13035.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..1810 /label=BBa_E0030 stem_loop 1830..1873 /label=stem_loop misc_feature 1819..1898 /label=BBa_B0010 terminator 1819..1898 /label=BBa_B0010 stem_loop 1914..1933 /label=T7 TE misc_feature 1940..1940 /label=stop polyA_site 1934..1947 /label=polya misc_feature 1907..1947 /label=BBa_B0012 terminator 1907..1947 /label=BBa_B0012 CDS 1956..2043 /label=end of LacI coding region (inactive) misc_feature 1956..2155 /label=BBa_R0010 misc_feature 2044..2081 /label=CAP binding site promoter 2092..2097 /label=-35 promoter 2116..2121 /label=-10 misc_feature 2121..2155 /label=LacI binding site misc_feature 2128..2128 /label=start promoter 1956..2155 /label=BBa_R0010 misc_feature 2168..2171 /label=conserved RBS 2164..2175 /label=BBa_B0034 CDS 2185..2801 /label=tetR misc_feature 2802..2835 /label=SsrA misc_feature 2842..2866 /label=Help:Barcodes CDS 2182..2841 /label=BBa_C0040 stem_loop 2886..2929 /label=stem_loop misc_feature 2875..2954 /label=BBa_B0010 terminator 2875..2954 /label=BBa_B0010 stem_loop 2970..2989 /label=T7 TE misc_feature 2996..2996 /label=stop polyA_site 2990..3003 /label=polya misc_feature 2963..3003 /label=BBa_B0012 terminator 2963..3003 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 1141 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 1201 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 1261 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca 1321 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 1381 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 1441 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 1501 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 1561 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 1621 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 1681 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca 1741 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 1801 caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1861 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1921 cttcgggtgg gcctttctgc gtttatatac tagagcaata cgcaaaccgc ctctccccgc 1981 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 2041 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 2101 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacatacta 2161 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 2221 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 2281 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 2341 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 2401 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 2461 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 2521 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 2581 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 2641 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 2701 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 2761 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 2821 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 2881 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2941 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3001 ata //