LOCUS BBa_I13037 5027 bp DNA linear 27-Sep-2024 DEFINITION Lux I/R Sender and Receiver w/ TetR regulation ACCESSION BBa_I13037 VERSION BBa_I13037.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 1816..1847 /label=LVA CDS 1237..1815 /label=luxI misc_feature 1237..1854 /label=BBa_C0061 misc_feature 1855..1879 /label=Help:Barcodes CDS 1237..1854 /label=BBa_C0061 stem_loop 1899..1942 /label=stem_loop misc_feature 1888..1967 /label=BBa_B0010 terminator 1888..1967 /label=BBa_B0010 stem_loop 1983..2002 /label=T7 TE misc_feature 2009..2009 /label=stop polyA_site 2003..2016 /label=polya misc_feature 1976..2016 /label=BBa_B0012 terminator 1976..2016 /label=BBa_B0012 misc_feature 2025..2043 /label=TetR 1 misc_feature 2025..2078 /label=BBa_R0040 promoter 2044..2049 /label=-35 misc_feature 2050..2068 /label=TetR 2 promoter 2067..2072 /label=-10 promoter 2025..2078 /label=BBa_R0040 misc_feature 2091..2094 /label=conserved RBS 2087..2098 /label=BBa_B0034 misc_feature 2105..2106 /label=prefix misc_feature 2278..2278 /label=T misc_feature 2596..2596 /label=A CDS 2105..2854 /label=luxR misc_feature 2105..2860 /label=BBa_C0062 misc_feature 2861..2885 /label=Help:Barcodes CDS 2105..2860 /label=BBa_C0062 stem_loop 2905..2948 /label=stem_loop misc_feature 2894..2973 /label=BBa_B0010 terminator 2894..2973 /label=BBa_B0010 stem_loop 2989..3008 /label=T7 TE misc_feature 3015..3015 /label=stop polyA_site 3009..3022 /label=polya misc_feature 2982..3022 /label=BBa_B0012 terminator 2982..3022 /label=BBa_B0012 misc_feature 3031..3050 /label=LuxR/HSL promoter 3050..3055 /label=-35 promoter 3072..3077 /label=-10 misc_feature 3083..3083 /label=start misc_feature 3031..3085 /label=BBa_R0062 promoter 3031..3085 /label=BBa_R0062 misc_feature 3098..3101 /label=conserved RBS 3094..3105 /label=BBa_B0034 CDS 3112..3834 /label=BBa_E0030 stem_loop 3854..3897 /label=stem_loop misc_feature 3843..3922 /label=BBa_B0010 terminator 3843..3922 /label=BBa_B0010 stem_loop 3938..3957 /label=T7 TE misc_feature 3964..3964 /label=stop polyA_site 3958..3971 /label=polya misc_feature 3931..3971 /label=BBa_B0012 terminator 3931..3971 /label=BBa_B0012 CDS 3980..4067 /label=end of LacI coding region (inactive) misc_feature 3980..4179 /label=BBa_R0010 misc_feature 4068..4105 /label=CAP binding site promoter 4116..4121 /label=-35 promoter 4140..4145 /label=-10 misc_feature 4145..4179 /label=LacI binding site misc_feature 4152..4152 /label=start promoter 3980..4179 /label=BBa_R0010 misc_feature 4192..4195 /label=conserved RBS 4188..4199 /label=BBa_B0034 CDS 4209..4825 /label=tetR misc_feature 4826..4859 /label=SsrA misc_feature 4866..4890 /label=Help:Barcodes CDS 4206..4865 /label=BBa_C0040 stem_loop 4910..4953 /label=stem_loop misc_feature 4899..4978 /label=BBa_B0010 terminator 4899..4978 /label=BBa_B0010 stem_loop 4994..5013 /label=T7 TE misc_feature 5020..5020 /label=stop polyA_site 5014..5027 /label=polya misc_feature 4987..5027 /label=BBa_B0012 terminator 4987..5027 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga ctataatgat aaaaaaatcg 1261 gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg ttatcaagtg 1321 tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc agatgagtat 1381 gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt aagtggatgc 1441 tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc tgaattgctt 1501 ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt tgctgtaggt 1561 aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact atttgaagct 1621 atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac atcaacagca 1681 atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga caaagaaatt 1741 catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga acagtttaaa 1801 aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta ataactctga 1861 tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 1921 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 1981 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 2041 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata 2101 ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg 2161 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga 2221 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct 2281 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga 2341 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa 2401 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat 2461 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc 2521 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc 2581 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc 2641 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag 2701 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac 2761 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt 2821 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag 2881 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 2941 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 3001 ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt ttacgcaaga 3061 aaatggtttg ttatagtcga ataaatacta gagaaagagg agaaatacta gatggtgagc 3121 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 3181 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 3241 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 3301 accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac 3361 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 3421 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 3481 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 3541 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 3601 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 3661 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 3721 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 3781 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag 3841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3961 ctgcgtttat atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 4021 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 4081 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 4141 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat 4201 actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg 4261 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc 4321 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga 4381 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt 4441 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag 4501 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct 4561 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc 4621 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg 4681 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc 4741 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa 4801 aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt 4861 aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa 4921 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4981 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //