LOCUS BBa_I13038 6037 bp DNA linear 22-Nov-2024 DEFINITION Lux I/R Sender and Receiver w/ TetR regulation ACCESSION BBa_I13038 VERSION BBa_I13038.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 1816..1847 /label=LVA CDS 1237..1815 /label=luxI misc_feature 1237..1854 /label=BBa_C0061 misc_feature 1855..1879 /label=Help:Barcodes CDS 1237..1854 /label=BBa_C0061 stem_loop 1899..1942 /label=stem_loop misc_feature 1888..1967 /label=BBa_B0010 terminator 1888..1967 /label=BBa_B0010 stem_loop 1983..2002 /label=T7 TE misc_feature 2009..2009 /label=stop polyA_site 2003..2016 /label=polya misc_feature 1976..2016 /label=BBa_B0012 terminator 1976..2016 /label=BBa_B0012 misc_feature 2025..2043 /label=TetR 1 misc_feature 2025..2078 /label=BBa_R0040 promoter 2044..2049 /label=-35 misc_feature 2050..2068 /label=TetR 2 promoter 2067..2072 /label=-10 promoter 2025..2078 /label=BBa_R0040 misc_feature 2091..2094 /label=conserved RBS 2087..2098 /label=BBa_B0034 misc_feature 2105..2106 /label=prefix misc_feature 2278..2278 /label=T misc_feature 2596..2596 /label=A CDS 2105..2854 /label=luxR misc_feature 2105..2860 /label=BBa_C0062 misc_feature 2861..2885 /label=Help:Barcodes CDS 2105..2860 /label=BBa_C0062 stem_loop 2905..2948 /label=stem_loop misc_feature 2894..2973 /label=BBa_B0010 terminator 2894..2973 /label=BBa_B0010 stem_loop 2989..3008 /label=T7 TE misc_feature 3015..3015 /label=stop polyA_site 3009..3022 /label=polya misc_feature 2982..3022 /label=BBa_B0012 terminator 2982..3022 /label=BBa_B0012 misc_feature 3031..3050 /label=LuxR/HSL promoter 3050..3055 /label=-35 promoter 3072..3077 /label=-10 misc_feature 3083..3083 /label=start misc_feature 3031..3085 /label=BBa_R0062 promoter 3031..3085 /label=BBa_R0062 misc_feature 3098..3101 /label=conserved RBS 3094..3105 /label=BBa_B0034 CDS 3112..3834 /label=BBa_E0030 stem_loop 3854..3897 /label=stem_loop misc_feature 3843..3922 /label=BBa_B0010 terminator 3843..3922 /label=BBa_B0010 stem_loop 3938..3957 /label=T7 TE misc_feature 3964..3964 /label=stop polyA_site 3958..3971 /label=polya misc_feature 3931..3971 /label=BBa_B0012 terminator 3931..3971 /label=BBa_B0012 promoter 5059..5189 /label=Pbad promoter 5022..5093 /label=PC misc_feature 3980..5058 /label=AraC promoter 3980..5189 /label=BBa_I0500 misc_feature 5202..5205 /label=conserved RBS 5198..5209 /label=BBa_B0034 CDS 5219..5835 /label=tetR misc_feature 5836..5869 /label=SsrA misc_feature 5876..5900 /label=Help:Barcodes CDS 5216..5875 /label=BBa_C0040 stem_loop 5920..5963 /label=stem_loop misc_feature 5909..5988 /label=BBa_B0010 terminator 5909..5988 /label=BBa_B0010 stem_loop 6004..6023 /label=T7 TE misc_feature 6030..6030 /label=stop polyA_site 6024..6037 /label=polya misc_feature 5997..6037 /label=BBa_B0012 terminator 5997..6037 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga ctataatgat aaaaaaatcg 1261 gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg ttatcaagtg 1321 tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc agatgagtat 1381 gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt aagtggatgc 1441 tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc tgaattgctt 1501 ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt tgctgtaggt 1561 aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact atttgaagct 1621 atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac atcaacagca 1681 atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga caaagaaatt 1741 catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga acagtttaaa 1801 aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta ataactctga 1861 tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 1921 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 1981 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 2041 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata 2101 ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg 2161 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga 2221 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct 2281 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga 2341 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa 2401 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat 2461 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc 2521 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc 2581 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc 2641 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag 2701 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac 2761 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt 2821 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag 2881 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 2941 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 3001 ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt ttacgcaaga 3061 aaatggtttg ttatagtcga ataaatacta gagaaagagg agaaatacta gatggtgagc 3121 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 3181 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 3241 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 3301 accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac 3361 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 3421 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 3481 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 3541 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 3601 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 3661 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 3721 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 3781 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag 3841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3961 ctgcgtttat atactagagt tatgacaact tgacggctac atcattcact ttttcttcac 4021 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat 4081 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc 4141 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc 4201 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga 4261 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc 4321 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta 4381 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg 4441 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa 4501 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg 4561 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat 4621 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct 4681 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca 4741 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca 4801 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac 4861 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc 4921 gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa 4981 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata 5041 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag 5101 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg 5161 tttctccata cccgtttttt tgggctagct actagagaaa gaggagaaat actagatgtc 5221 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 5281 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 5341 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 5401 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 5461 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 5521 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 5581 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 5641 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 5701 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 5761 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 5821 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 5881 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 5941 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 6001 actggctcac cttcgggtgg gcctttctgc gtttata //