LOCUS       BBa_I13100              1930 bp    DNA     linear       25-Nov-2024
DEFINITION  c1 Self-coupled Inverter
ACCESSION   BBa_I13100
VERSION     BBa_I13100.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             79..786
                     /label=cI lambda
     misc_feature    787..819
                     /label=LVA
     misc_feature    826..850
                     /label=Help:Barcodes
     CDS             76..825
                     /label=BBa_C0051
     stem_loop       870..913
                     /label=stem_loop
     misc_feature    859..938
                     /label=BBa_B0010
     terminator      859..938
                     /label=BBa_B0010
     stem_loop       954..973
                     /label=T7 TE
     misc_feature    980..980
                     /label=stop
     polyA_site      974..987
                     /label=polya
     misc_feature    947..987
                     /label=BBa_B0012
     terminator      947..987
                     /label=BBa_B0012
     promoter        1033..1038
                     /label=-10
     promoter        1010..1015
                     /label=-35
     misc_feature    1020..1036
                     /label=OR1
     misc_feature    996..1012
                     /label=OR2
     misc_feature    996..1044
                     /label=BBa_R0051
     promoter        996..1044
                     /label=BBa_R0051
     misc_feature    1057..1060
                     /label=conserved
     RBS             1053..1064
                     /label=BBa_B0034
     CDS             1071..1793
                     /label=BBa_E0030
     stem_loop       1813..1856
                     /label=stem_loop
     misc_feature    1802..1881
                     /label=BBa_B0010
     terminator      1802..1881
                     /label=BBa_B0010
     stem_loop       1897..1916
                     /label=T7 TE
     misc_feature    1923..1923
                     /label=stop
     polyA_site      1917..1930
                     /label=polya
     misc_feature    1890..1930
                     /label=BBa_B0012
     terminator      1890..1930
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac
      121 gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa
      181 tctgtcgcag acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc
      241 aatgcattaa atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa
      301 gaatttagcc cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag
      361 ccgtcactta gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc
      421 tcacctgagc ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa
      481 aaagccagtg attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca
      541 ggctccaagc caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt
      601 gagccaggtg atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg
      661 atcagggata gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca
      721 tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg
      781 tttggcgctg caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag
      841 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      961 cttcgggtgg gcctttctgc gtttatatac tagagtaaca ccgtgcgtgt tgactatttt
     1021 acctctggcg gtgataatgg ttgctactag agaaagagga gaaatactag atggtgagca
     1081 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1141 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1201 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1261 ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact
     1321 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1381 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1441 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1501 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1561 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1621 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1681 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1741 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga
     1801 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1861 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1921 tgcgtttata
//