LOCUS       BBa_I13105              1853 bp    DNA     linear       22-Dec-2024
DEFINITION  Low output control (tet QPI)
ACCESSION   BBa_I13105
VERSION     BBa_I13105.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    70..73
                     /label=conserved
     RBS             64..77
                     /label=BBa_B0031
     CDS             87..703
                     /label=tetR
     misc_feature    704..737
                     /label=SsrA
     misc_feature    744..768
                     /label=Help:Barcodes
     CDS             84..743
                     /label=BBa_C0040
     stem_loop       788..831
                     /label=stem_loop
     misc_feature    777..856
                     /label=BBa_B0010
     terminator      777..856
                     /label=BBa_B0010
     stem_loop       872..891
                     /label=T7 TE
     misc_feature    898..898
                     /label=stop
     polyA_site      892..905
                     /label=polya
     misc_feature    865..905
                     /label=BBa_B0012
     terminator      865..905
                     /label=BBa_B0012
     misc_feature    914..932
                     /label=TetR 1
     misc_feature    914..967
                     /label=BBa_R0040
     promoter        933..938
                     /label=-35
     misc_feature    939..957
                     /label=TetR 2
     promoter        956..961
                     /label=-10
     promoter        914..967
                     /label=BBa_R0040
     misc_feature    980..983
                     /label=conserved
     RBS             976..987
                     /label=BBa_B0034
     CDS             994..1716
                     /label=BBa_E0030
     stem_loop       1736..1779
                     /label=stem_loop
     misc_feature    1725..1804
                     /label=BBa_B0010
     terminator      1725..1804
                     /label=BBa_B0010
     stem_loop       1820..1839
                     /label=T7 TE
     misc_feature    1846..1846
                     /label=stop
     polyA_site      1840..1853
                     /label=polya
     misc_feature    1813..1853
                     /label=BBa_B0012
     terminator      1813..1853
                     /label=BBa_B0012
     misc_feature    64..1853
                     /label=BBa_I13023
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaacctac tagatgtcca gattagataa aagtaaagtg attaacagcg
      121 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      181 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      241 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      301 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      361 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      421 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      481 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      541 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      601 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
      661 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa
      721 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
      961 tgagcactac tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc
     1021 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1081 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1141 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     1201 caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg
     1261 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1321 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1381 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1441 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1501 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1561 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     1621 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1681 atcactctcg gcatggacga gctgtacaag taataatact agagccaggc atcaaataaa
     1741 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1801 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//