LOCUS       BBa_I13106              1851 bp    DNA     linear       25-Nov-2024
DEFINITION  Low output control (tet QPI, Elowitz RBS)
ACCESSION   BBa_I13106
VERSION     BBa_I13106.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..701
                     /label=tetR
     misc_feature    702..735
                     /label=SsrA
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             82..741
                     /label=BBa_C0040
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
     misc_feature    912..930
                     /label=TetR 1
     misc_feature    912..965
                     /label=BBa_R0040
     promoter        931..936
                     /label=-35
     misc_feature    937..955
                     /label=TetR 2
     promoter        954..959
                     /label=-10
     promoter        912..965
                     /label=BBa_R0040
     misc_feature    978..981
                     /label=conserved
     RBS             974..985
                     /label=BBa_B0034
     CDS             992..1714
                     /label=BBa_E0030
     stem_loop       1734..1777
                     /label=stem_loop
     misc_feature    1723..1802
                     /label=BBa_B0010
     terminator      1723..1802
                     /label=BBa_B0010
     stem_loop       1818..1837
                     /label=T7 TE
     misc_feature    1844..1844
                     /label=stop
     polyA_site      1838..1851
                     /label=polya
     misc_feature    1811..1851
                     /label=BBa_B0012
     terminator      1811..1851
                     /label=BBa_B0012
     misc_feature    64..1851
                     /label=BBa_I13022
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
      121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
      661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
      721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg
      961 agcactacta gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac
     1021 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1081 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1141 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     1201 atgcttcgcc cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc
     1261 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1321 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1381 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1441 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1501 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1561 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     1621 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1681 cactctcggc atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac
     1741 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1801 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//