LOCUS       BBa_I13109              1877 bp    DNA     linear       25-Nov-2024
DEFINITION  Low output control (c2 QPI, Elowitz RBS)
ACCESSION   BBa_I13109
VERSION     BBa_I13109.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..728
                     /label=cII p22
     misc_feature    729..761
                     /label=LVA
     misc_feature    762..767
                     /label=stop
     misc_feature    81..767
                     /label=BBa_C0053
     misc_feature    768..792
                     /label=Help:Barcodes
     CDS             81..767
                     /label=BBa_C0053
     stem_loop       812..855
                     /label=stem_loop
     misc_feature    801..880
                     /label=BBa_B0010
     terminator      801..880
                     /label=BBa_B0010
     stem_loop       896..915
                     /label=T7 TE
     misc_feature    922..922
                     /label=stop
     polyA_site      916..929
                     /label=polya
     misc_feature    889..929
                     /label=BBa_B0012
     terminator      889..929
                     /label=BBa_B0012
     misc_feature    938..940
                     /label=OR3
     misc_feature    948..965
                     /label=OR2
     misc_feature    971..988
                     /label=OR1
     promoter        955..960
                     /label=-35
     promoter        945..950
                     /label=-35
     promoter        967..972
                     /label=-10
     misc_feature    938..991
                     /label=BBa_R0053
     promoter        938..991
                     /label=BBa_R0053
     misc_feature    1004..1007
                     /label=conserved
     RBS             1000..1011
                     /label=BBa_B0034
     CDS             1018..1740
                     /label=BBa_E0030
     stem_loop       1760..1803
                     /label=stem_loop
     misc_feature    1749..1828
                     /label=BBa_B0010
     terminator      1749..1828
                     /label=BBa_B0010
     stem_loop       1844..1863
                     /label=T7 TE
     misc_feature    1870..1870
                     /label=stop
     polyA_site      1864..1877
                     /label=polya
     misc_feature    1837..1877
                     /label=BBa_B0012
     terminator      1837..1877
                     /label=BBa_B0012
     misc_feature    63..1877
                     /label=BBa_I13020
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaatacac aattgatggg tgagcgtatt cgcgctcgaa
      121 gaaaaaaact caagattaga caagccgctc ttggtaagat ggtgggagtg tctaatgttg
      181 caatatcgca atgggagcgc tcggagactg agccaaatgg ggagaacctg ttggcacttt
      241 cgaaggctct tcagtgctcc cctgactatt tgctgaaagg agatttaagc cagacaaacg
      301 ttgcctatca tagtaggcat gagccaagag gatcataccc tcttatcagt tgggtaagcg
      361 cagggcaatg gatggaagct gtagaacctt atcacaagcg cgcgatagag aactggcacg
      421 acaccactgt agattgttca gaagattcat tttggcttga tgtccaaggt gactctatga
      481 cagcaccggc agggttaagc attccagaag gaatgataat tctggttgat cccgaagtcg
      541 aaccaagaaa cggcaagctg gttgttgcaa aattagaagg tgaaaacgag gccacattca
      601 aaaaattagt tatggatgca ggccgaaagt ttttaaaacc attaaaccca caatatccga
      661 tgatagaaat caacggaaac tgcaaaatca ttggcgtagt tgttgacgca aaactcgcaa
      721 atcttccagc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct
      781 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      841 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      901 accttcgggt gggcctttct gcgtttatat actagagaat aaacttgact aaagattcct
      961 ttagtagata atttaagtgt tctttaattt ctactagaga aagaggagaa atactagatg
     1021 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1081 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1141 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1201 gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg
     1261 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1321 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1381 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1441 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1501 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1561 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1621 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1681 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa
     1741 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1801 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1861 gcctttctgc gtttata
//