LOCUS       BBa_I13110              1850 bp    DNA     linear       22-May-2024
DEFINITION  Tet Self-coupled Inverter
ACCESSION   BBa_I13110
VERSION     BBa_I13110.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..700
                     /label=tetR
     misc_feature    701..734
                     /label=SsrA
     misc_feature    741..765
                     /label=Help:Barcodes
     CDS             81..740
                     /label=BBa_C0040
     stem_loop       785..828
                     /label=stem_loop
     misc_feature    774..853
                     /label=BBa_B0010
     terminator      774..853
                     /label=BBa_B0010
     stem_loop       869..888
                     /label=T7 TE
     misc_feature    895..895
                     /label=stop
     polyA_site      889..902
                     /label=polya
     misc_feature    862..902
                     /label=BBa_B0012
     terminator      862..902
                     /label=BBa_B0012
     misc_feature    911..929
                     /label=TetR 1
     misc_feature    911..964
                     /label=BBa_R0040
     promoter        930..935
                     /label=-35
     misc_feature    936..954
                     /label=TetR 2
     promoter        953..958
                     /label=-10
     promoter        911..964
                     /label=BBa_R0040
     misc_feature    977..980
                     /label=conserved
     RBS             973..984
                     /label=BBa_B0034
     CDS             991..1713
                     /label=BBa_E0030
     stem_loop       1733..1776
                     /label=stem_loop
     misc_feature    1722..1801
                     /label=BBa_B0010
     terminator      1722..1801
                     /label=BBa_B0010
     stem_loop       1817..1836
                     /label=T7 TE
     misc_feature    1843..1843
                     /label=stop
     polyA_site      1837..1850
                     /label=polya
     misc_feature    1810..1850
                     /label=BBa_B0012
     terminator      1810..1850
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
      121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca
      781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      901 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      961 gcactactag agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc
     1021 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1081 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1141 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa
     1201 tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc
     1261 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1321 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1381 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1441 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1501 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1561 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     1621 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1681 actctcggca tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg
     1741 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1801 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//