LOCUS       BBa_I13111              2321 bp    DNA     linear       22-Nov-2024
DEFINITION  Low output control (lac QPI, B0031 RBS)
ACCESSION   BBa_I13111
VERSION     BBa_I13111.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     misc_feature    1172..1210
                     /label=LVA
     CDS             83..1210
                     /label=lacI-LVA
     misc_feature    83..1210
                     /label=BBa_C0012
     misc_feature    1211..1235
                     /label=Help:Barcodes
     CDS             83..1210
                     /label=BBa_C0012
     stem_loop       1255..1298
                     /label=stem_loop
     misc_feature    1244..1323
                     /label=BBa_B0010
     terminator      1244..1323
                     /label=BBa_B0010
     stem_loop       1339..1358
                     /label=T7 TE
     misc_feature    1365..1365
                     /label=stop
     polyA_site      1359..1372
                     /label=polya
     misc_feature    1332..1372
                     /label=BBa_B0012
     terminator      1332..1372
                     /label=BBa_B0012
     terminator      1244..1372
                     /label=BBa_B0015
     misc_feature    1383..1399
                     /label=lac O1
     promoter        1400..1405
                     /label=-35
     misc_feature    1406..1422
                     /label=lac O1
     promoter        1423..1428
                     /label=-10
     misc_feature    1381..1434
                     /label=BBa_R0011
     promoter        1381..1434
                     /label=BBa_R0011
     misc_feature    63..1435
                     /label=BBa_Q01121
     misc_feature    1448..1451
                     /label=conserved
     RBS             1444..1455
                     /label=BBa_B0034
     CDS             1462..2184
                     /label=BBa_E0030
     stem_loop       2204..2247
                     /label=stem_loop
     misc_feature    2193..2272
                     /label=BBa_B0010
     terminator      2193..2272
                     /label=BBa_B0010
     stem_loop       2288..2307
                     /label=T7 TE
     misc_feature    2314..2314
                     /label=stop
     polyA_site      2308..2321
                     /label=polya
     misc_feature    2281..2321
                     /label=BBa_B0012
     terminator      2281..2321
                     /label=BBa_B0012
     misc_feature    1444..2321
                     /label=BBa_E0430
     misc_feature    63..2321
                     /label=BBa_I13024
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaacctact agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc
      121 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
      181 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
      241 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
      301 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
      361 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
      421 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
      481 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
      541 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
      601 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
      661 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
      721 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
      781 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
      841 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
      901 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
      961 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     1021 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     1081 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1141 acgacaggtt tcccgactgg aaagcgggca ggctgcaaac gacgaaaact acgctttagt
     1201 agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa
     1261 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1321 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1381 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
     1441 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
     1501 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1561 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1621 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
     1681 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1741 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1801 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1861 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1921 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1981 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2041 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     2101 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2161 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca
     2221 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2281 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//