LOCUS       BBa_I13114              2394 bp    DNA     linear       22-Nov-2024
DEFINITION  High Output control (lac QPI)
ACCESSION   BBa_I13114
VERSION     BBa_I13114.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     stem_loop       1328..1371
                     /label=stem_loop
     misc_feature    1317..1396
                     /label=BBa_B0010
     terminator      1317..1396
                     /label=BBa_B0010
     stem_loop       1412..1431
                     /label=T7 TE
     misc_feature    1438..1438
                     /label=stop
     polyA_site      1432..1445
                     /label=polya
     misc_feature    1405..1445
                     /label=BBa_B0012
     terminator      1405..1445
                     /label=BBa_B0012
     terminator      1317..1445
                     /label=BBa_B0015
     misc_feature    142..145
                     /label=conserved
     RBS             138..149
                     /label=BBa_B0034
     misc_feature    1245..1283
                     /label=LVA
     CDS             156..1283
                     /label=lacI-LVA
     misc_feature    156..1283
                     /label=BBa_C0012
     misc_feature    1284..1308
                     /label=Help:Barcodes
     CDS             156..1283
                     /label=BBa_C0012
     stem_loop       1328..1371
                     /label=stem_loop
     misc_feature    1317..1396
                     /label=BBa_B0010
     terminator      1317..1396
                     /label=BBa_B0010
     stem_loop       1412..1431
                     /label=T7 TE
     misc_feature    1438..1438
                     /label=stop
     polyA_site      1432..1445
                     /label=polya
     misc_feature    1405..1445
                     /label=BBa_B0012
     terminator      1405..1445
                     /label=BBa_B0012
     terminator      1317..1445
                     /label=BBa_B0015
     misc_feature    1456..1472
                     /label=lac O1
     promoter        1473..1478
                     /label=-35
     misc_feature    1479..1495
                     /label=lac O1
     promoter        1496..1501
                     /label=-10
     misc_feature    1454..1507
                     /label=BBa_R0011
     promoter        1454..1507
                     /label=BBa_R0011
     misc_feature    138..1508
                     /label=BBa_Q04121
     misc_feature    1521..1524
                     /label=conserved
     RBS             1517..1528
                     /label=BBa_B0034
     CDS             1535..2257
                     /label=BBa_E0030
     stem_loop       2277..2320
                     /label=stem_loop
     misc_feature    2266..2345
                     /label=BBa_B0010
     terminator      2266..2345
                     /label=BBa_B0010
     stem_loop       2361..2380
                     /label=T7 TE
     misc_feature    2387..2387
                     /label=stop
     polyA_site      2381..2394
                     /label=polya
     misc_feature    2354..2394
                     /label=BBa_B0012
     terminator      2354..2394
                     /label=BBa_B0012
     misc_feature    1517..2394
                     /label=BBa_E0430
     misc_feature    138..2394
                     /label=BBa_I13021
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagaaa gaggagaaat actagatggt gaatgtgaaa ccagtaacgt
      181 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
      241 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
      301 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
      361 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
      421 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
      481 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
      541 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
      601 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
      661 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
      721 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
      781 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
      841 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
      901 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
      961 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     1021 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     1081 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     1141 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1201 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa
     1261 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag
     1321 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1381 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1441 ttatatacta gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac
     1501 tgagcacata ctagagaaag aggagaaata ctagatggtg agcaagggcg aggagctgtt
     1561 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1621 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1681 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
     1741 gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca agtccgccat
     1801 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1861 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1921 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1981 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2041 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2101 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     2161 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2221 gatcactctc ggcatggacg agctgtacaa gtaataatac tagagccagg catcaaataa
     2281 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2341 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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