LOCUS       BBa_I13115              1925 bp    DNA     linear       22-Dec-2024
DEFINITION  High output control (tet QPI)
ACCESSION   BBa_I13115
VERSION     BBa_I13115.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    142..145
                     /label=conserved
     RBS             138..149
                     /label=BBa_B0034
     CDS             159..775
                     /label=tetR
     misc_feature    776..809
                     /label=SsrA
     misc_feature    816..840
                     /label=Help:Barcodes
     CDS             156..815
                     /label=BBa_C0040
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
     misc_feature    986..1004
                     /label=TetR 1
     misc_feature    986..1039
                     /label=BBa_R0040
     promoter        1005..1010
                     /label=-35
     misc_feature    1011..1029
                     /label=TetR 2
     promoter        1028..1033
                     /label=-10
     promoter        986..1039
                     /label=BBa_R0040
     misc_feature    1052..1055
                     /label=conserved
     RBS             1048..1059
                     /label=BBa_B0034
     CDS             1066..1788
                     /label=BBa_E0030
     stem_loop       1808..1851
                     /label=stem_loop
     misc_feature    1797..1876
                     /label=BBa_B0010
     terminator      1797..1876
                     /label=BBa_B0010
     stem_loop       1892..1911
                     /label=T7 TE
     misc_feature    1918..1918
                     /label=stop
     polyA_site      1912..1925
                     /label=polya
     misc_feature    1885..1925
                     /label=BBa_B0012
     terminator      1885..1925
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag
      181 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
      241 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
      301 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
      361 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
      421 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
      481 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
      541 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
      601 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
      661 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
      721 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg
      781 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag
     1021 tgatagagat actgagcact actagagaaa gaggagaaat actagatggt gagcaagggc
     1081 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1141 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1201 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     1261 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc
     1321 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1381 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1501 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1561 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1621 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1681 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1741 accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata ctagagccag
     1801 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1861 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1921 ttata
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