LOCUS BBa_I13205 4239 bp DNA linear 13-May-2024 DEFINITION HSL/aiiA test construct ACCESSION BBa_I13205 VERSION BBa_I13205.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 CDS 1185..1907 /label=BBa_E0030 stem_loop 1927..1970 /label=stem_loop misc_feature 1916..1995 /label=BBa_B0010 terminator 1916..1995 /label=BBa_B0010 stem_loop 2011..2030 /label=T7 TE misc_feature 2037..2037 /label=stop polyA_site 2031..2044 /label=polya misc_feature 2004..2044 /label=BBa_B0012 terminator 2004..2044 /label=BBa_B0012 promoter 3132..3262 /label=Pbad promoter 3095..3166 /label=PC misc_feature 2053..3131 /label=AraC promoter 2053..3262 /label=BBa_I0500 misc_feature 3275..3278 /label=conserved RBS 3271..3282 /label=BBa_B0034 misc_feature 3289..3291 /label=start misc_feature 4072..4077 /label=2 misc_feature 4039..4071 /label=LVA CDS 3289..4038 /label=aiiA misc_feature 3289..4077 /label=BBa_C0060 misc_feature 4078..4102 /label=Help:Barcodes CDS 3289..4077 /label=BBa_C0060 stem_loop 4122..4165 /label=stem_loop misc_feature 4111..4190 /label=BBa_B0010 terminator 4111..4190 /label=BBa_B0010 stem_loop 4206..4225 /label=T7 TE misc_feature 4232..4232 /label=stop polyA_site 4226..4239 /label=polya misc_feature 4199..4239 /label=BBa_B0012 terminator 4199..4239 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatggtg agcaagggcg 1201 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 1261 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 1321 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg 1381 gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca 1441 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 1501 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 1561 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 1621 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 1681 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 1741 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt 1801 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 1861 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac tagagccagg 1921 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1981 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2041 tatatactag agttatgaca acttgacggc tacatcattc actttttctt cacaaccggc 2101 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg 2161 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag 2221 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg 2281 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc 2341 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg 2401 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg 2461 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat 2521 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc 2581 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa 2641 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc 2701 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt 2761 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg 2821 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg 2881 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat 2941 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg 3001 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct 3061 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg 3121 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt 3181 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc 3241 atacccgttt ttttgggcta gctactagag aaagaggaga aatactagat gacagtaaag 3301 aagctttatt tcgtcccagc aggtcgttgt atgttggatc attcgtctgt taatagtaca 3361 ttaacaccag gagaattatt agacttaccg gtttggtgtt atcttttgga gactgaagaa 3421 ggacctattt tagtagatac aggtatgcca gaaagtgcag ttaataatga aggtcttttt 3481 aacggtacat ttgtcgaagg gcaggtttta ccgaaaatga ctgaagaaga tagaatcgtg 3541 aatattttaa aacgggttgg ttatgagccg gaagaccttc tttatattat tagttctcac 3601 ttgcattttg atcatgcagg aggaaatggc gcttttataa atacaccaat cattgtacag 3661 cgtgctgaat atgaggcggc gcagcatagc gaagaatatt tgaaagaatg tatattgccg 3721 aatttaaact acaaaatcat tgaaggtgat tatgaagtcg taccaggagt tcaattattg 3781 catacaccag gccatactcc agggcatcaa tcgctattaa ttgagacaga aaaatccggt 3841 cctgtattat taacgattga tgcatcgtat acgaaagaga attttgaaaa tgaagtgcca 3901 tttgcgggat ttgattcaga attagcttta tcttcaatta aacgtttaaa agaagtggtg 3961 atgaaagaga agccgattgt tttctttgga catgatatag agcaggaaag gggatgtaaa 4021 gtgttccctg aatatatagc tgcaaacgac gaaaactacg ctttagtagc ttaataacgc 4081 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 4141 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 4201 acactggctc accttcgggt gggcctttct gcgtttata //