LOCUS       BBa_I13209              1917 bp    DNA     linear       14-May-2024
DEFINITION  Relaxation Oscillator Test Construct (I13202.B0015.I0460)
ACCESSION   BBa_I13209
VERSION     BBa_I13209.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..666
                     /label=LuxI
     CDS             82..666
                     /label=BBa_C0161
     stem_loop       686..729
                     /label=stem_loop
     misc_feature    675..754
                     /label=BBa_B0010
     terminator      675..754
                     /label=BBa_B0010
     stem_loop       770..789
                     /label=T7 TE
     misc_feature    796..796
                     /label=stop
     polyA_site      790..803
                     /label=polya
     misc_feature    763..803
                     /label=BBa_B0012
     terminator      763..803
                     /label=BBa_B0012
     misc_feature    1..803
                     /label=BBa_I13202
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
     terminator      812..940
                     /label=BBa_B0015
     misc_feature    953..956
                     /label=conserved
     RBS             949..960
                     /label=BBa_B0034
     misc_feature    967..969
                     /label=start
     misc_feature    1750..1755
                     /label=2
     misc_feature    1717..1749
                     /label=LVA
     CDS             967..1716
                     /label=aiiA
     misc_feature    967..1755
                     /label=BBa_C0060
     misc_feature    1756..1780
                     /label=Help:Barcodes
     CDS             967..1755
                     /label=BBa_C0060
     stem_loop       1800..1843
                     /label=stem_loop
     misc_feature    1789..1868
                     /label=BBa_B0010
     terminator      1789..1868
                     /label=BBa_B0010
     stem_loop       1884..1903
                     /label=T7 TE
     misc_feature    1910..1910
                     /label=stop
     polyA_site      1904..1917
                     /label=polya
     misc_feature    1877..1917
                     /label=BBa_B0012
     terminator      1877..1917
                     /label=BBa_B0012
     misc_feature    949..1917
                     /label=BBa_I0460
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      721 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      781 gggtgggcct ttctgcgttt atatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa agaggagaaa
      961 tactagatga cagtaaagaa gctttatttc gtcccagcag gtcgttgtat gttggatcat
     1021 tcgtctgtta atagtacatt aacaccagga gaattattag acttaccggt ttggtgttat
     1081 cttttggaga ctgaagaagg acctatttta gtagatacag gtatgccaga aagtgcagtt
     1141 aataatgaag gtctttttaa cggtacattt gtcgaagggc aggttttacc gaaaatgact
     1201 gaagaagata gaatcgtgaa tattttaaaa cgggttggtt atgagccgga agaccttctt
     1261 tatattatta gttctcactt gcattttgat catgcaggag gaaatggcgc ttttataaat
     1321 acaccaatca ttgtacagcg tgctgaatat gaggcggcgc agcatagcga agaatatttg
     1381 aaagaatgta tattgccgaa tttaaactac aaaatcattg aaggtgatta tgaagtcgta
     1441 ccaggagttc aattattgca tacaccaggc catactccag ggcatcaatc gctattaatt
     1501 gagacagaaa aatccggtcc tgtattatta acgattgatg catcgtatac gaaagagaat
     1561 tttgaaaatg aagtgccatt tgcgggattt gattcagaat tagctttatc ttcaattaaa
     1621 cgtttaaaag aagtggtgat gaaagagaag ccgattgttt tctttggaca tgatatagag
     1681 caggaaaggg gatgtaaagt gttccctgaa tatatagctg caaacgacga aaactacgct
     1741 ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa
     1801 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1861 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//