LOCUS BBa_I13210 5724 bp DNA linear 25-Nov-2024 DEFINITION Lux/Cin Relaxation Oscillator ACCESSION BBa_I13210 VERSION BBa_I13210.1 FEATURES Location/Qualifiers misc_feature 1..220 /label=unidentified/uncharacterized CinR dependent stem_loop 35..74 /label=stem_loop stem_loop 91..136 /label=stem_loop promoter 1..225 /label=BBa_R0078 misc_feature 238..241 /label=conserved RBS 234..245 /label=BBa_B0034 misc_feature 252..254 /label=start misc_feature 1035..1040 /label=2 misc_feature 1002..1034 /label=LVA CDS 252..1001 /label=aiiA misc_feature 252..1040 /label=BBa_C0060 misc_feature 1041..1065 /label=Help:Barcodes CDS 252..1040 /label=BBa_C0060 stem_loop 1085..1128 /label=stem_loop misc_feature 1074..1153 /label=BBa_B0010 terminator 1074..1153 /label=BBa_B0010 stem_loop 1169..1188 /label=T7 TE misc_feature 1195..1195 /label=stop polyA_site 1189..1202 /label=polya misc_feature 1162..1202 /label=BBa_B0012 terminator 1162..1202 /label=BBa_B0012 misc_feature 234..1202 /label=BBa_I0460 misc_feature 1211..1430 /label=unidentified/uncharacterized CinR dependent stem_loop 1245..1284 /label=stem_loop stem_loop 1301..1346 /label=stem_loop promoter 1211..1435 /label=BBa_R0078 misc_feature 1448..1451 /label=conserved RBS 1444..1455 /label=BBa_B0034 CDS 1462..2184 /label=BBa_E0020 stem_loop 2204..2247 /label=stem_loop misc_feature 2193..2272 /label=BBa_B0010 terminator 2193..2272 /label=BBa_B0010 stem_loop 2288..2307 /label=T7 TE misc_feature 2314..2314 /label=stop polyA_site 2308..2321 /label=polya misc_feature 2281..2321 /label=BBa_B0012 terminator 2281..2321 /label=BBa_B0012 misc_feature 1444..2321 /label=BBa_E0420 promoter 2330..3352 /label=BBa_I13201 misc_feature 3365..3368 /label=conserved RBS 3361..3372 /label=BBa_B0034 misc_feature 3958..3989 /label=LVA CDS 3379..3957 /label=luxI misc_feature 3379..3996 /label=BBa_C0061 misc_feature 3997..4021 /label=Help:Barcodes CDS 3379..3996 /label=BBa_C0061 misc_feature 4034..4037 /label=conserved RBS 4030..4041 /label=BBa_B0034 CDS 4048..4770 /label=cinR misc_feature 4771..4803 /label=LVA misc_feature 4810..4834 /label=Help:Barcodes CDS 4048..4834 /label=BBa_C0077 misc_feature 4847..4850 /label=conserved RBS 4843..4854 /label=BBa_B0034 CDS 4861..5523 /label=CinI misc_feature 5524..5556 /label=LVA misc_feature 5563..5587 /label=Help:Barcodes CDS 4861..5562 /label=BBa_C0076 stem_loop 5607..5650 /label=stem_loop misc_feature 5596..5675 /label=BBa_B0010 terminator 5596..5675 /label=BBa_B0010 stem_loop 5691..5710 /label=T7 TE misc_feature 5717..5717 /label=stop polyA_site 5711..5724 /label=polya misc_feature 5684..5724 /label=BBa_B0012 terminator 5684..5724 /label=BBa_B0012 misc_feature 4843..5724 /label=BBa_F1760 ORIGIN 1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc 61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac 121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga 181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctacta gagaaagagg 241 agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt tgtatgttgg 301 atcattcgtc tgttaatagt acattaacac caggagaatt attagactta ccggtttggt 361 gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg ccagaaagtg 421 cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt ttaccgaaaa 481 tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag ccggaagacc 541 ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat ggcgctttta 601 taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat agcgaagaat 661 atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt gattatgaag 721 tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat caatcgctat 781 taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg tatacgaaag 841 agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct ttatcttcaa 901 ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt ggacatgata 961 tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac gacgaaaact 1021 acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca 1081 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1141 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1201 tatactagag ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc 1261 tttcagattc gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac 1321 cgctgcacac ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg 1381 gaatttccga tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctacta 1441 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 1501 cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag 1561 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 1621 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc 1681 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 1741 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 1801 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 1861 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc 1921 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag 1981 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 2041 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 2101 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 2161 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca 2221 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 2281 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt taatttttaa 2341 agtatgggca atcaattgct cctgttaaaa ttgctttaga aatactttgg cagcggtttg 2401 ttgtattgag tttcatttgc gcattggtta aatggaaagt gacagtacgc tcactgcagc 2461 ctaatatttt tgaaatatcc caagagcttt ttccttcgca tgcccacgct aaacattctt 2521 tttctctttt ggttaaatcg ttgtttgatt tattatttgc tatatttatt tttcgataat 2581 tatcaactag agaaggaaca attaatggta tgttcataca cgcatgtaaa aataaactat 2641 ctatatagtt gtctttttct gaatgtgcaa aactaagcat tccgaagcca ttgttagccg 2701 tatgaatagg gaaactaaac ccagtgataa gacctgatgt tttcgcttct ttaattacat 2761 ttggagattt tttatttaca gcattgtttt caaatatatt ccaattaatt ggtgaatgat 2821 tggagttaga ataatctact ataggatcat attttattaa attagcgtca tcataatatt 2881 gcctccattt tttagggtaa ttatctagaa ttgaaatatc agatttaacc atagaatgag 2941 gataaatgat cgcgagtaaa taatattcac aatgtaccat tttagtcata tcagataagc 3001 attgattaat atcattattg cttctacaag ctttaatttt attaattatt ctgtatgtgt 3061 cgtcggcatt tatgtttttc atacccatct ctttatcctt acctattgtt tgtcgcaagt 3121 tttgcgtgtt atatatcatt aaaacggtaa tggattgaca tcctattgtt tgtcgcaagt 3181 tttgcgtgtt atatatcatt aaaacggtaa tagattgaca tttgattcta ataaattgga 3241 tttttgtcac actattatat cgcttgaaat acaattgttt aacataagta cctgtaggat 3301 cgtacaggtt tacgcaagaa aatggtttgt tatagtcgat taaacgcaag ggtactagag 3361 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 3421 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 3481 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 3541 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 3601 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 3661 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 3721 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 3781 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 3841 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 3901 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 3961 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 4021 ctactagaga aagaggagaa atactagatg attgagaata cctatagcga aaagttcgag 4081 tccgcgttcg aacagatcaa ggcggcggcc aacgtggatg ccgccatccg tattctccag 4141 gcggaatata acctcgattt cgtcacctac catctcgccc agacgatcgc gagcaagatc 4201 gattcgccct tcgtgcgcac cacctatccg gatgcctggg tttcccgcta cctcctcaac 4261 agctatgtga aggtcgatcc gatcgtcaag cagggcttcg aacgccagct gcccttcgac 4321 tggagcgagg tcgaaccgac gccggaggcc tatgccatgc tggtcgacgc ccagaaacac 4381 ggcatcggtg gcaatggcta ctccatcccc gtcgccgaca aggcgcagcg ccgcgccctg 4441 ctgtcgctga atgcccgtat accggccgac gaatggaccg agctcgtgcg ccgctgccgc 4501 aacgagtgga tcgagatcgc ccatctgatc caccgcaagg ccgtctatga gctgcatggc 4561 gaaaacgatc cggtgccggc attgtcgccg cgcgagatcg agtgtctgca ctggaccgcc 4621 ctcggcaagg attacaagga tatttcggtc atcctgggca tatcagagca taccacacgc 4681 gattacctga agaccgcccg cttcaagctc ggctgcgcca cgatctcggc cgccgcgtcg 4741 cgggctgttc aattgcgcat catcaatccc gctgcaaacg acgaaaacta cgctttagta 4801 gcttaataac gctgatagtg ctagtgtaga tcgctactag agaaagagga gaaatactag 4861 atgttcgtta tcatccaggc tcacgaatac cagaaatacg ctgctgttct ggaccagatg 4921 ttccgtctgc gtaaaaaagt tttcgctgac accctgtgct gggacgttcc ggttatcggt 4981 ccgtacgaac gtgactccta cgactccctg gctccggctt acctggtttg gtgcaacgac 5041 tcccgtaccc gtctgtacgg tggtatgcgt ctgatgccga ccaccggtcc gaccctgctg 5101 tacgacgttt tccgtgaaac cttcccggac gctgctgacc tgatcgctcc gggtatctgg 5161 gaaggtaccc gtatgtgcat cgacgaagaa gctatcgcta aagacttccc ggaaatcgac 5221 gctggtcgtg ctttctccat gatgctgctg gctctgtgcg aatgcgctct ggaccacggt 5281 atccacacca tgatctccaa ctacgaaccg tacctgaaac gtgtttacaa acgtgctggt 5341 gctgaagttg aagaactggg tcgtgctgac ggttacggta aatacccggt ttgctgcggt 5401 gctttcgaag tttccgaccg tgttctgcgt aaaatgcgtg ctgctctggg tctgaccctg 5461 ccgctgtacg ttcgtcacgt tccggctcgt tccgttgtta cccagttcct ggaaatggct 5521 gctgctgcta acgacgaaaa ctacgctctg gttgcttaat aaccctgata gtgctagtgt 5581 agatccctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 5641 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 5701 cgggtgggcc tttctgcgtt tata //