BBa_I13210
type: ComponentDefinition
persistent identity: BBa I13210
display id: BBa_I13210
version: 1
was generated by: iGEM to SBOL conversion
description: Lux/Cin Relaxation Oscillator
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:33Z
owned by: james
owned by: myers
mutable description: Reporter tripped by CinR-HSL Threshold, tripped by Lux-HSL Threshold. CinR-HSL also promotes production of aiiA. This should break the thresholds and reset the system.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: SMUG Cell-Cell Signalling: Jay
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_C0077
component: BBa_B0034
component: BBa_F1760
component: BBa_R0078
component: BBa_I0460
component: BBa_I13201
component: BBa_E0420
component: BBa_C0061
sequence annotation: BBa_R0078
sequence annotation: BBa_I0460
sequence annotation: BBa_E0420
sequence annotation: BBa_I13201
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_C0077
sequence annotation: BBa_F1760
sequence: https://synbiohub.org/public/igem/BBa_I13210_sequence/1

BBa_C0077
type: Component
persistent identity: component2237186
display id: component2237186
version: 1
top level: BBa_I13210
definition: cinr
access: public

BBa_B0034
type: Component
persistent identity: component2237182
display id: component2237182
version: 1
top level: BBa_I13210
definition: BBa_B0034
access: public

BBa_F1760
type: Component
persistent identity: component2237199
display id: component2237199
version: 1
top level: BBa_I13210
definition: BBa_F1760
access: public

BBa_R0078
type: Component
persistent identity: component2237144
display id: component2237144
version: 1
top level: BBa_I13210
definition: cinR
access: public

BBa_R0078
type: Component
persistent identity: component2237163
display id: component2237163
version: 1
top level: BBa_I13210
definition: cinR
access: public

BBa_I0460
type: Component
persistent identity: component2237159
display id: component2237159
version: 1
top level: BBa_I13210
definition: BBa_I0460
access: public

BBa_I13201
type: Component
persistent identity: component2237174
display id: component2237174
version: 1
top level: BBa_I13210
definition: LuxR/P
access: public

BBa_E0420
type: Component
persistent identity: component2237173
display id: component2237173
version: 1
top level: BBa_I13210
definition: BBa_E0420
access: public

BBa_C0061
type: Component
persistent identity: component2237178
display id: component2237178
version: 1
top level: BBa_I13210
definition: luxI
access: public

BBa_B0034
type: Component
persistent identity: component2237176
display id: component2237176
version: 1
top level: BBa_I13210
definition: BBa_B0034
access: public

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation2237144
display id: annotation2237144
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237144/range2237144/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I13210/annotation2237144/range2237144/1
type: Range
persistent identity: range2237144
display id: range2237144
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 1
end: 225
orientation: inline

BBa_I0460
type: SequenceAnnotation
persistent identity: annotation2237159
display id: annotation2237159
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237159/range2237159/1
component: BBa_I0460

https://synbiohub.org/public/igem/BBa_I13210/annotation2237159/range2237159/1
type: Range
persistent identity: range2237159
display id: range2237159
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 234
end: 1202
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation2237163
display id: annotation2237163
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237163/range2237163/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I13210/annotation2237163/range2237163/1
type: Range
persistent identity: range2237163
display id: range2237163
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 1211
end: 1435
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2237173
display id: annotation2237173
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237173/range2237173/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13210/annotation2237173/range2237173/1
type: Range
persistent identity: range2237173
display id: range2237173
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 1444
end: 2321
orientation: inline

BBa_I13201
type: SequenceAnnotation
persistent identity: annotation2237174
display id: annotation2237174
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237174/range2237174/1
component: BBa_I13201

https://synbiohub.org/public/igem/BBa_I13210/annotation2237174/range2237174/1
type: Range
persistent identity: range2237174
display id: range2237174
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 2330
end: 3352
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2237176
display id: annotation2237176
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237176/range2237176/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13210/annotation2237176/range2237176/1
type: Range
persistent identity: range2237176
display id: range2237176
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 3361
end: 3372
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2237178
display id: annotation2237178
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237178/range2237178/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_I13210/annotation2237178/range2237178/1
type: Range
persistent identity: range2237178
display id: range2237178
version: 1
top level: BBa_I13210
direction: direction/forward
start: 3379
end: 3996
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2237182
display id: annotation2237182
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237182/range2237182/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13210/annotation2237182/range2237182/1
type: Range
persistent identity: range2237182
display id: range2237182
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 4030
end: 4041
orientation: inline

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation2237186
display id: annotation2237186
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237186/range2237186/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_I13210/annotation2237186/range2237186/1
type: Range
persistent identity: range2237186
display id: range2237186
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 4048
end: 4834
orientation: inline

BBa_F1760
type: SequenceAnnotation
persistent identity: annotation2237199
display id: annotation2237199
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237199/range2237199/1
component: BBa_F1760

https://synbiohub.org/public/igem/BBa_I13210/annotation2237199/range2237199/1
type: Range
persistent identity: range2237199
display id: range2237199
version: 1
top level: BBa_I13210
direction: direction/reverse
start: 4843
end: 5724
orientation: inline

LuxR/P
type: ComponentDefinition
persistent identity: BBa I13201
display id: BBa_I13201
version: 1
was generated by: iGEM to SBOL conversion
description: Natural Lux Promoter Preceded by LuxR
created: 2004-07-28T11:00:00Z
modified: 2015-08-31T04:07:33Z
mutable provenance: pTK1
owned by: james
owned by: myers
mutable description: I13200 with the inverted LuxR sequence preceding it. Taken directly from the natural system.
discontinued: true
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: mit
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I13201_sequence/1

BBa_F1760
type: ComponentDefinition
persistent identity: BBa F1760
display id: BBa_F1760
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC14HSL Sender Device
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: Sender device which accepts PoPS input and produces an enzyme CinI. This enzyme produces the 3OC14HSL signaling molecule.
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: mit
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_B0012
component: BBa_B0010
component: BBa_C0076
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0076
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_F1760_sequence/1

BBa_B0012
type: Component
persistent identity: component943362
display id: component943362
version: 1
top level: BBa_F1760
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component943352
display id: component943352
version: 1
top level: BBa_F1760
definition: BBa_B0010
access: public

BBa_C0076
type: Component
persistent identity: component943346
display id: component943346
version: 1
top level: BBa_F1760
definition: cinI
access: public

BBa_B0034
type: Component
persistent identity: component943336
display id: component943336
version: 1
top level: BBa_F1760
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943336
display id: annotation943336
version: 1
top level: BBa_F1760
location: https://synbiohub.org/public/igem/BBa_F1760/annotation943336/range943336/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F1760/annotation943336/range943336/1
type: Range
persistent identity: range943336
display id: range943336
version: 1
top level: BBa_F1760
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0076
type: SequenceAnnotation
persistent identity: annotation943346
display id: annotation943346
version: 1
top level: BBa_F1760
location: https://synbiohub.org/public/igem/BBa_F1760/annotation943346/range943346/1
component: BBa_C0076

https://synbiohub.org/public/igem/BBa_F1760/annotation943346/range943346/1
type: Range
persistent identity: range943346
display id: range943346
version: 1
top level: BBa_F1760
direction: direction/forward
start: 19
end: 720
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943352
display id: annotation943352
version: 1
top level: BBa_F1760
location: https://synbiohub.org/public/igem/BBa_F1760/annotation943352/range943352/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F1760/annotation943352/range943352/1
type: Range
persistent identity: range943352
display id: range943352
version: 1
top level: BBa_F1760
direction: direction/forward
start: 754
end: 833
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943362
display id: annotation943362
version: 1
top level: BBa_F1760
location: https://synbiohub.org/public/igem/BBa_F1760/annotation943362/range943362/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1760/annotation943362/range943362/1
type: Range
persistent identity: range943362
display id: range943362
version: 1
top level: BBa_F1760
direction: direction/forward
start: 842
end: 882
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (cinR and HSL regulated)
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
top level: cinR
direction: direction/forward
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
top level: cinR
direction: direction/forward
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
top level: cinR
direction: direction/forward
start: 91
end: 136
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_I0460
type: ComponentDefinition
persistent identity: BBa I0460
display id: BBa_I0460
version: 1
was generated by: iGEM to SBOL conversion
description: aiiA Device (B0034.C0060.B0015)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: true
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_C0060
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0060
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0460_sequence/1

BBa_B0012
type: Component
persistent identity: component943127
display id: component943127
version: 1
top level: BBa_I0460
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component943117
display id: component943117
version: 1
top level: BBa_I0460
definition: BBa_B0010
access: public

BBa_C0060
type: Component
persistent identity: component943109
display id: component943109
version: 1
top level: BBa_I0460
definition: aiiA
access: public

BBa_B0034
type: Component
persistent identity: component943094
display id: component943094
version: 1
top level: BBa_I0460
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943094
display id: annotation943094
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943094/range943094/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0460/annotation943094/range943094/1
type: Range
persistent identity: range943094
display id: range943094
version: 1
top level: BBa_I0460
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation943109
display id: annotation943109
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943109/range943109/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I0460/annotation943109/range943109/1
type: Range
persistent identity: range943109
display id: range943109
version: 1
top level: BBa_I0460
direction: direction/forward
start: 19
end: 807
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943117
display id: annotation943117
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943117/range943117/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0460/annotation943117/range943117/1
type: Range
persistent identity: range943117
display id: range943117
version: 1
top level: BBa_I0460
direction: direction/forward
start: 841
end: 920
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943127
display id: annotation943127
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943127/range943127/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0460/annotation943127/range943127/1
type: Range
persistent identity: range943127
display id: range943127
version: 1
top level: BBa_I0460
direction: direction/forward
start: 929
end: 969
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: iGEM to SBOL conversion
description: cinR activator from Rhizobium leguminosarum (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cinR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
top level: cinr
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
top level: cinr
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
top level: cinr
direction: direction/forward
start: 763
end: 787
orientation: inline

BBa_E0420
type: ComponentDefinition
persistent identity: BBa E0420
display id: BBa_E0420
version: 1
was generated by: iGEM to SBOL conversion
description: ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Standard CFP output device w/o LVA tag.
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0420_sequence/1

BBa_B0034
type: Component
persistent identity: component942780
display id: component942780
version: 1
top level: BBa_E0420
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component942797
display id: component942797
version: 1
top level: BBa_E0420
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component942787
display id: component942787
version: 1
top level: BBa_E0420
definition: BBa_B0010
access: public

BBa_E0020
type: Component
persistent identity: component942782
display id: component942782
version: 1
top level: BBa_E0420
definition: ecfp
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942780
display id: annotation942780
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
type: Range
persistent identity: range942780
display id: range942780
version: 1
top level: BBa_E0420
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation942782
display id: annotation942782
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
type: Range
persistent identity: range942782
display id: range942782
version: 1
top level: BBa_E0420
direction: direction/forward
start: 19
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942787
display id: annotation942787
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
type: Range
persistent identity: range942787
display id: range942787
version: 1
top level: BBa_E0420
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942797
display id: annotation942797
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
type: Range
persistent identity: range942797
display id: range942797
version: 1
top level: BBa_E0420
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cinI
type: ComponentDefinition
persistent identity: BBa C0076
display id: BBa_C0076
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cinI codes for an autoinducer synthetase which utilizes SAM to make O3-C14:1-HSL. In complex with O3-C14:1-HSL, CinR (BBa_C0077) binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tga -> tAaTAA
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CinI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0076_sequence/1

CinI
type: SequenceAnnotation
persistent identity: annotation301035
display id: annotation301035
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
type: Range
persistent identity: range301035
display id: range301035
version: 1
top level: cinI
direction: direction/forward
start: 1
end: 663
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306586
display id: annotation306586
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
type: Range
persistent identity: range306586
display id: range306586
version: 1
top level: cinI
direction: direction/forward
start: 664
end: 696
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214001
display id: annotation2214001
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
type: Range
persistent identity: range2214001
display id: range2214001
version: 1
top level: cinI
direction: direction/forward
start: 703
end: 727
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: <genbank>AF196486</genbank> from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: aiiA
sequence annotation: BBa_C0060
sequence annotation: start
sequence annotation: LVA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 750
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 789
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
top level: aiiA
direction: direction/forward
start: 751
end: 783
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
top level: aiiA
direction: direction/forward
start: 784
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
top level: aiiA
direction: direction/forward
start: 790
end: 814
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

https://synbiohub.org/public/igem/BBa_I13210_sequence/1
type: Sequence
persistent identity: BBa I13210 sequence
display id: BBa_I13210_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13201_sequence/1
type: Sequence
persistent identity: BBa I13201 sequence
display id: BBa_I13201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaatttttaaagtatgggcaatcaattgctcctgttaaaattgctttagaaatactttggcagcggtttgttgtattgagtttcatttgcgcattggttaaatggaaagtgacagtacgctcactgcagcctaatatttttgaaatatcccaagagctttttccttcgcatgcccacgctaaacattctttttctcttttggttaaatcgttgtttgatttattatttgctatatttatttttcgataattatcaactagagaaggaacaattaatggtatgttcatacacgcatgtaaaaataaactatctatatagttgtctttttctgaatgtgcaaaactaagcattccgaagccattgttagccgtatgaatagggaaactaaacccagtgataagacctgatgttttcgcttctttaattacatttggagattttttatttacagcattgttttcaaatatattccaattaattggtgaatgattggagttagaataatctactataggatcatattttattaaattagcgtcatcataatattgcctccattttttagggtaattatctagaattgaaatatcagatttaaccatagaatgaggataaatgatcgcgagtaaataatattcacaatgtaccattttagtcatatcagataagcattgattaatatcattattgcttctacaagctttaattttattaattattctgtatgtgtcgtcggcatttatgtttttcatacccatctctttatccttacctattgtttgtcgcaagttttgcgtgttatatatcattaaaacggtaatggattgacatcctattgtttgtcgcaagttttgcgtgttatatatcattaaaacggtaatagattgacatttgattctaataaattggatttttgtcacactattatatcgcttgaaatacaattgtttaacataagtacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgattaaacgcaaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_F1760_sequence/1
type: Sequence
persistent identity: BBa F1760 sequence
display id: BBa_F1760_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0078_sequence/1
type: Sequence
persistent identity: BBa R0078 sequence
display id: BBa_R0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0460_sequence/1
type: Sequence
persistent identity: BBa I0460 sequence
display id: BBa_I0460_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0077_sequence/1
type: Sequence
persistent identity: BBa C0077 sequence
display id: BBa_C0077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0420_sequence/1
type: Sequence
persistent identity: BBa E0420 sequence
display id: BBa_E0420_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0060_sequence/1
type: Sequence
persistent identity: BBa C0060 sequence
display id: BBa_C0060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0076_sequence/1
type: Sequence
persistent identity: BBa C0076 sequence
display id: BBa_C0076_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z