LOCUS       BBa_I13271              1003 bp    DNA     linear       22-Dec-2024
DEFINITION  PoPS->PoPS amplifier (RhlR-based, HSL driven)
ACCESSION   BBa_I13271
VERSION     BBa_I13271.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..741
                     /label=rhlR
     misc_feature    742..774
                     /label=LVA
     misc_feature    781..805
                     /label=Help:Barcodes
     CDS             19..780
                     /label=BBa_C0071
     stem_loop       825..868
                     /label=stem_loop
     misc_feature    814..893
                     /label=BBa_B0010
     terminator      814..893
                     /label=BBa_B0010
     stem_loop       909..928
                     /label=T7 TE
     misc_feature    935..935
                     /label=stop
     polyA_site      929..942
                     /label=polya
     misc_feature    902..942
                     /label=BBa_B0012
     terminator      902..942
                     /label=BBa_B0012
     misc_feature    951..970
                     /label=RhlR
     promoter        969..974
                     /label=-35
     promoter        992..997
                     /label=-10
     misc_feature    1003..1003
                     /label=start
     promoter        951..1003
                     /label=BBa_R0071
ORIGIN
        1 aaagaggaga aatactagat gaggaatgac ggaggctttt tgctgtggtg ggacggtttg
       61 cgtagcgaga tgcagccgat ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa
      121 gtgcggcgcc tgggcttcga ttactacgcc tatggcgtgc gccacacgat tcccttcacc
      181 cggccgaaga ccgaggtcca tggcacctat cccaaggcct ggctggagcg ataccagatg
      241 cagaactacg gggccgtgga tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg
      301 gtctggagcg acagcctgtt cgaccagagc cggatgctct ggaacgaggc tcgcgattgg
      361 ggcctctgtg tcggcgcgac cttgccgatc cgcgcgccga acaatttgct cagcgtgctt
      421 tccgtggcgc gcgaccagca gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg
      481 ctgcgttgca tgatcgagtt gctgacccag aagctgaccg acctggagca tccgatgctg
      541 atgtccaacc cggtctgcct gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac
      601 ggcaagagtt ccggggaaat cgccatcatc ctgagcatct ccgagagcac ggtgaacttc
      661 caccacaaga acatccagaa gaagttcgac gcgccgaaca agacgctggc tgccgcctac
      721 gccgcggcgc tgggtctcat cgctgcaaac gacgaaaact acgctttagt agcttaataa
      781 cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc
      841 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      901 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tcctgtgaaa
      961 tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttc
//