LOCUS       BBa_I13303              1066 bp    DNA     linear       21-May-2024
DEFINITION  R0011.I13271 Lac-Regulated Rhl Amplifier
ACCESSION   BBa_I13303
VERSION     BBa_I13303.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..804
                     /label=rhlR
     misc_feature    805..837
                     /label=LVA
     misc_feature    844..868
                     /label=Help:Barcodes
     CDS             82..843
                     /label=BBa_C0071
     stem_loop       888..931
                     /label=stem_loop
     misc_feature    877..956
                     /label=BBa_B0010
     terminator      877..956
                     /label=BBa_B0010
     stem_loop       972..991
                     /label=T7 TE
     misc_feature    998..998
                     /label=stop
     polyA_site      992..1005
                     /label=polya
     misc_feature    965..1005
                     /label=BBa_B0012
     terminator      965..1005
                     /label=BBa_B0012
     misc_feature    1014..1033
                     /label=RhlR
     promoter        1032..1037
                     /label=-35
     promoter        1055..1060
                     /label=-10
     misc_feature    1066..1066
                     /label=start
     promoter        1014..1066
                     /label=BBa_R0071
     misc_feature    64..1066
                     /label=BBa_I13271
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt
      121 ttgcgtagcg agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag
      181 gaagtgcggc gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc
      241 acccggccga agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag
      301 atgcagaact acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg
      361 gtggtctgga gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat
      421 tggggcctct gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg
      481 ctttccgtgg cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg
      541 cggctgcgtt gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg
      601 ctgatgtcca acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc
      661 gacggcaaga gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac
      721 ttccaccaca agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc
      781 tacgccgcgg cgctgggtct catcgctgca aacgacgaaa actacgcttt agtagcttaa
      841 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtcctgtg
     1021 aaatctggca gttaccgtta gctttcgaat tggctaaaaa gtgttc
//