LOCUS       BBa_I13308              1237 bp    DNA     linear       22-Dec-2024
DEFINITION  R0040.I13277 Tet-Regulated Cin Amplifier
ACCESSION   BBa_I13308
VERSION     BBa_I13308.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=cinR
     misc_feature    804..836
                     /label=LVA
     misc_feature    843..867
                     /label=Help:Barcodes
     CDS             81..842
                     /label=BBa_C0077
     stem_loop       887..930
                     /label=stem_loop
     misc_feature    876..955
                     /label=BBa_B0010
     terminator      876..955
                     /label=BBa_B0010
     stem_loop       971..990
                     /label=T7 TE
     misc_feature    997..997
                     /label=stop
     polyA_site      991..1004
                     /label=polya
     misc_feature    964..1004
                     /label=BBa_B0012
     terminator      964..1004
                     /label=BBa_B0012
     misc_feature    1013..1232
                     /label=unidentified/uncharacterized CinR dependent
     stem_loop       1047..1086
                     /label=stem_loop
     stem_loop       1103..1148
                     /label=stem_loop
     promoter        1013..1237
                     /label=BBa_R0078
     misc_feature    63..1237
                     /label=BBa_I13277
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgattgaga atacctatag cgaaaagttc gagtccgcgt
      121 tcgaacagat caaggcggcg gccaacgtgg atgccgccat ccgtattctc caggcggaat
      181 ataacctcga tttcgtcacc taccatctcg cccagacgat cgcgagcaag atcgattcgc
      241 ccttcgtgcg caccacctat ccggatgcct gggtttcccg ctacctcctc aacagctatg
      301 tgaaggtcga tccgatcgtc aagcagggct tcgaacgcca gctgcccttc gactggagcg
      361 aggtcgaacc gacgccggag gcctatgcca tgctggtcga cgcccagaaa cacggcatcg
      421 gtggcaatgg ctactccatc cccgtcgccg acaaggcgca gcgccgcgcc ctgctgtcgc
      481 tgaatgcccg tataccggcc gacgaatgga ccgagctcgt gcgccgctgc cgcaacgagt
      541 ggatcgagat cgcccatctg atccaccgca aggccgtcta tgagctgcat ggcgaaaacg
      601 atccggtgcc ggcattgtcg ccgcgcgaga tcgagtgtct gcactggacc gccctcggca
      661 aggattacaa ggatatttcg gtcatcctgg gcatatcaga gcataccaca cgcgattacc
      721 tgaagaccgc ccgcttcaag ctcggctgcg ccacgatctc ggccgccgcg tcgcgggctg
      781 ttcaattgcg catcatcaat cccgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
      901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agccctttgt
     1021 gcgtccaaac ggacgcacgg cgctctaaag cgggtcgcga tctttcagat tcgctcctcg
     1081 cgctttcagt ctttgttttg gcgcatgtcg ttatcgcaaa accgctgcac acttttgcgc
     1141 gacatgctct gatccccctc atctgggggg gcctatctga gggaatttcc gatccggctc
     1201 gcctgaacca ttctgctttc cacgaacttg aaaacgc
//