##gff-version 3
##sequence-region BBa_I13309 1 1952
BBa_I13309	.	engineered_region	1075	1952	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_I13309	.	terminator	1824	1903	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_I13309	.	stem_loop	1835	1878	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13309	.	engineered_region	1824	1903	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13309	.	ribosome_entry_site	1075	1086	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_I13309	.	conserved_region	1079	1082	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13309	.	terminator	1912	1952	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_I13309	.	polyA_site	1939	1952	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13309	.	engineered_region	1912	1952	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13309	.	stem_loop	1919	1938	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13309	.	stop_codon	1945	1945	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13309	.	CDS	1093	1815	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_I13309	.	engineered_region	1	1066	.	+	0	ID=BBa_I13303;Name=BBa_I13303
BBa_I13309	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I13303
BBa_I13309	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I13309	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I13309	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I13309	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I13309	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I13309	.	engineered_region	64	1066	.	+	0	ID=BBa_I13271;Name=BBa_I13271;Parent=BBa_I13303
BBa_I13309	.	promoter	1014	1066	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_I13271
BBa_I13309	.	non_covalent_binding_site	1014	1033	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_I13309	.	promoter	1055	1060	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_I13309	.	promoter	1032	1037	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_I13309	.	start_codon	1066	1066	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_I13309	.	terminator	965	1005	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13271
BBa_I13309	.	polyA_site	992	1005	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13309	.	engineered_region	965	1005	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13309	.	stem_loop	972	991	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13309	.	stop_codon	998	998	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13309	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13271
BBa_I13309	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13309	.	CDS	82	843	.	+	0	ID=BBa_C0071;Name=BBa_C0071;Parent=BBa_I13271
BBa_I13309	.	CDS	82	804	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_I13309	.	engineered_tag	844	868	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_I13309	.	tag	805	837	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_I13309	.	terminator	877	956	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13271
BBa_I13309	.	stem_loop	888	931	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13309	.	engineered_region	877	956	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I13309
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccaggg
cgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccc
ttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacgggg
ccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccg
gatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctc
agcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgca
tgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcg
cgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagc
acggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcgg
cgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcact
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctg
gcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagatggtgagcaaggg
cgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtcc
ggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccct
ggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgactt
cttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgc
gccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaaca
tcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaa
ggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatc
ggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagc
gcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata