LOCUS       BBa_I13310              1893 bp    DNA     linear       21-May-2024
DEFINITION  I13304.E0430 Tet-Regulated Rhl Amplifier with YFP Reporter
ACCESSION   BBa_I13310
VERSION     BBa_I13310.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..809
                     /label=BBa_C0171
     stem_loop       829..872
                     /label=stem_loop
     misc_feature    818..897
                     /label=BBa_B0010
     terminator      818..897
                     /label=BBa_B0010
     stem_loop       913..932
                     /label=T7 TE
     misc_feature    939..939
                     /label=stop
     polyA_site      933..946
                     /label=polya
     misc_feature    906..946
                     /label=BBa_B0012
     terminator      906..946
                     /label=BBa_B0012
     misc_feature    955..974
                     /label=RhlR
     promoter        973..978
                     /label=-35
     promoter        996..1001
                     /label=-10
     misc_feature    1007..1007
                     /label=start
     promoter        955..1007
                     /label=BBa_R0071
     misc_feature    1020..1023
                     /label=conserved
     RBS             1016..1027
                     /label=BBa_B0034
     CDS             1034..1756
                     /label=BBa_E0030
     stem_loop       1776..1819
                     /label=stem_loop
     misc_feature    1765..1844
                     /label=BBa_B0010
     terminator      1765..1844
                     /label=BBa_B0010
     stem_loop       1860..1879
                     /label=T7 TE
     misc_feature    1886..1886
                     /label=stop
     polyA_site      1880..1893
                     /label=polya
     misc_feature    1853..1893
                     /label=BBa_B0012
     terminator      1853..1893
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt
      121 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg
      181 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca
      241 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga
      301 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg
      361 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt
      421 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc
      481 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc
      541 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc
      601 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg
      661 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact
      721 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct
      781 acgccgcggc gctgggtctc atctaataat actagagcca ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcctgt
      961 gaaatctggc agttaccgtt agctttcgaa ttggctaaaa agtgttctac tagagaaaga
     1021 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1081 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1141 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1201 gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc
     1261 cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1321 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1381 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1441 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1501 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1561 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1621 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     1681 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1741 gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1801 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1861 gctcaccttc gggtgggcct ttctgcgttt ata
//