LOCUS       BBa_I13311              1992 bp    DNA     linear       18-May-2024
DEFINITION  I13305.E0430 Lac-Regulated Las Amplifier with YFP Reporter
ACCESSION   BBa_I13311
VERSION     BBa_I13311.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..804
                     /label=BBa_C0179
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    995..1012
                     /label=OP2
     misc_feature    1055..1074
                     /label=OP1
     promoter        1066..1071
                     /label=-35
     promoter        1089..1094
                     /label=-10
     promoter        950..1106
                     /label=BBa_R0079
     misc_feature    64..1106
                     /label=BBa_I13279
     misc_feature    1..1106
                     /label=BBa_I13305
     misc_feature    1119..1122
                     /label=conserved
     RBS             1115..1126
                     /label=BBa_B0034
     CDS             1133..1855
                     /label=BBa_E0030
     stem_loop       1875..1918
                     /label=stem_loop
     misc_feature    1864..1943
                     /label=BBa_B0010
     terminator      1864..1943
                     /label=BBa_B0010
     stem_loop       1959..1978
                     /label=T7 TE
     misc_feature    1985..1985
                     /label=stop
     polyA_site      1979..1992
                     /label=polya
     misc_feature    1952..1992
                     /label=BBa_B0012
     terminator      1952..1992
                     /label=BBa_B0012
     misc_feature    1115..1992
                     /label=BBa_E0430
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggccttg gttgacggtt ttcttgagct ggaacgctca
      121 agtggaaaat tggagtggag cgccatcctc cagaagatgg cgagcgacct tggattctcg
      181 aagatcctgt tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc
      241 ggcaactacc cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac
      301 ccgacggtca gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac
      361 cagacgcgaa agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg
      421 ctgaccatgc cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa
      481 gcggaaaacc gggccgaggc caaccgtttc atagagtcgg tcctgccgac cctgtggatg
      541 ctcaaggact acgcactgca aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa
      601 ccggtggttc tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc
      661 agttgggaga tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga
      721 aatattcggc ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat
      781 ttgggtctta ttactctcta ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagg cccctcgctg
      961 agcgcgtccc ggagctgggg gcaacctagc tgccacctgc ttttctgcta gctattccag
     1021 cgaaaacata cagatttccg gcgaaatcaa ggctacctgc cagttctggc aggtttggcc
     1081 gcgggttctt tttggtacac gaaagctact agagaaagag gagaaatact agatggtgag
     1141 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1201 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1261 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1321 caccttcggc tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga
     1381 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1441 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1501 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1561 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1621 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1681 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1741 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1801 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aataatacta
     1861 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1921 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1981 tctgcgttta ta
//