LOCUS       BBa_I13403              2964 bp    DNA     linear       22-Nov-2024
DEFINITION  YFP + Pbad CFP
ACCESSION   BBa_I13403
VERSION     BBa_I13403.1  
FEATURES             Location/Qualifiers
     CDS             1..723
                     /label=BBa_E0030
     stem_loop       743..786
                     /label=stem_loop
     misc_feature    732..811
                     /label=BBa_B0010
     terminator      732..811
                     /label=BBa_B0010
     stem_loop       827..846
                     /label=T7 TE
     misc_feature    853..853
                     /label=stop
     polyA_site      847..860
                     /label=polya
     misc_feature    820..860
                     /label=BBa_B0012
     terminator      820..860
                     /label=BBa_B0012
     promoter        1948..2078
                     /label=Pbad
     promoter        1911..1982
                     /label=PC
     misc_feature    869..1947
                     /label=AraC
     promoter        869..2078
                     /label=BBa_I0500
     misc_feature    2091..2094
                     /label=conserved
     RBS             2087..2098
                     /label=BBa_B0034
     CDS             2105..2827
                     /label=BBa_E0020
     stem_loop       2847..2890
                     /label=stem_loop
     misc_feature    2836..2915
                     /label=BBa_B0010
     terminator      2836..2915
                     /label=BBa_B0010
     stem_loop       2931..2950
                     /label=T7 TE
     misc_feature    2957..2957
                     /label=stop
     polyA_site      2951..2964
                     /label=polya
     misc_feature    2924..2964
                     /label=BBa_B0012
     terminator      2924..2964
                     /label=BBa_B0012
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag
      241 ctgcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      781 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      841 tgggcctttc tgcgtttata tactagagtt atgacaactt gacggctaca tcattcactt
      901 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
      961 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
     1021 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
     1081 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
     1141 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
     1201 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
     1261 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
     1321 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
     1381 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
     1441 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
     1501 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
     1561 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     1621 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     1681 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     1741 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
     1801 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc
     1861 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
     1921 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
     1981 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
     2041 tctctactgt ttctccatac ccgttttttt gggctagcta ctagagaaag aggagaaata
     2101 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2161 ggacggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2221 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2281 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
     2341 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2401 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2461 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2521 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa
     2581 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2641 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2701 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2761 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2821 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2881 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2941 cgggtgggcc tttctgcgtt tata
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