LOCUS       BBa_I13404              2979 bp    DNA     linear       30-Apr-2024
DEFINITION  RBS.GFP + PBad CFP
ACCESSION   BBa_I13404
VERSION     BBa_I13404.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     promoter        1963..2093
                     /label=Pbad
     promoter        1926..1997
                     /label=PC
     misc_feature    884..1962
                     /label=AraC
     promoter        884..2093
                     /label=BBa_I0500
     misc_feature    2106..2109
                     /label=conserved
     RBS             2102..2113
                     /label=BBa_B0034
     CDS             2120..2842
                     /label=BBa_E0020
     stem_loop       2862..2905
                     /label=stem_loop
     misc_feature    2851..2930
                     /label=BBa_B0010
     terminator      2851..2930
                     /label=BBa_B0010
     stem_loop       2946..2965
                     /label=T7 TE
     misc_feature    2972..2972
                     /label=stop
     polyA_site      2966..2979
                     /label=polya
     misc_feature    2939..2979
                     /label=BBa_B0012
     terminator      2939..2979
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttatgac aacttgacgg
      901 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
      961 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     1021 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     1081 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     1141 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     1201 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     1261 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     1321 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     1381 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     1441 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     1501 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     1561 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     1621 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     1681 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     1741 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     1801 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
     1861 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     1921 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     1981 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     2041 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agctactaga
     2101 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2161 catcctggtc gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg
     2221 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2281 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg
     2341 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2401 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2461 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2521 cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg tctatatcac
     2581 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     2641 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2701 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     2761 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2821 ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga aaggctcagt
     2881 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2941 acactggctc accttcgggt gggcctttct gcgtttata
//