LOCUS       BBa_I13410              2863 bp    DNA     linear       17-May-2024
DEFINITION  Screening Plasmid Test Construct v2
ACCESSION   BBa_I13410
VERSION     BBa_I13410.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1219..1231
                     /label=B0032
     misc_feature    1238..1960
                     /label=E0020
     misc_feature    1219..1976
                     /label=BBa_I13411
     RBS             1985..1997
                     /label=RBS-3\Weak
     RBS             1991..1994
                     /label=RBS
     misc_feature    1985..1997
                     /label=BBa_B0032
     RBS             1985..1997
                     /label=BBa_B0032
     CDS             2004..2726
                     /label=BBa_E0030
     stem_loop       2746..2789
                     /label=stem_loop
     misc_feature    2735..2814
                     /label=BBa_B0010
     terminator      2735..2814
                     /label=BBa_B0010
     stem_loop       2830..2849
                     /label=T7 TE
     misc_feature    2856..2856
                     /label=stop
     polyA_site      2850..2863
                     /label=polya
     misc_feature    2823..2863
                     /label=BBa_B0012
     terminator      2823..2863
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggaaa gtactagatg gtgagcaagg gcgaggagct
     1261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
     1321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
     1441 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag
     1681 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     1741 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1921 cgggatcact ctcggcatgg acgagctgta caagtaataa aagctttacc atggattact
     1981 agagtcacac aggaaagtac tagatggtga gcaagggcga ggagctgttc accggggtgg
     2041 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2101 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2161 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg
     2221 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct
     2281 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2341 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2401 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2461 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2521 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2581 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     2641 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2701 gcatggacga gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct
     2761 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2821 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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