LOCUS       BBa_I13507               861 bp    DNA     linear       22-Dec-2024
DEFINITION  Screening plasmid intermediate
ACCESSION   BBa_I13507
VERSION     BBa_I13507.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..693
                     /label=mrfp1
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             19..699
                     /label=BBa_E1010
     stem_loop       744..787
                     /label=stem_loop
     misc_feature    733..812
                     /label=BBa_B0010
     terminator      733..812
                     /label=BBa_B0010
     stem_loop       828..847
                     /label=T7 TE
     misc_feature    854..854
                     /label=stop
     polyA_site      848..861
                     /label=polya
     misc_feature    821..861
                     /label=BBa_B0012
     terminator      821..861
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
       61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      721 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      841 gtgggccttt ctgcgtttat a
//