LOCUS       BBa_I13512              2887 bp    DNA     linear       03-May-2024
DEFINITION  Screening plasmid 1
ACCESSION   BBa_I13512
VERSION     BBa_I13512.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1231
                     /label=RBS-3\Weak
     RBS             1225..1228
                     /label=RBS
     misc_feature    1219..1231
                     /label=BBa_B0032
     RBS             1219..1231
                     /label=BBa_B0032
     CDS             1238..1912
                     /label=mrfp1
     misc_feature    1919..1943
                     /label=Help:Barcodes
     CDS             1238..1918
                     /label=BBa_E1010
     misc_feature    1952..1957
                     /label=Future Eco site
     misc_feature    1959..1964
                     /label=BbsI site
     misc_feature    1992..2003
                     /label=BstXI site
     misc_feature    1995..2000
                     /label=Future Pst site
     misc_feature    1952..2003
                     /label=BBa_I13452
     RBS             2012..2024
                     /label=RBS-3\Weak
     RBS             2018..2021
                     /label=RBS
     misc_feature    2012..2024
                     /label=BBa_B0032
     RBS             2012..2024
                     /label=BBa_B0032
     CDS             2031..2750
                     /label=GFP protein
     CDS             2031..2750
                     /label=BBa_E0040
     stem_loop       2770..2813
                     /label=stem_loop
     misc_feature    2759..2838
                     /label=BBa_B0010
     terminator      2759..2838
                     /label=BBa_B0010
     stem_loop       2854..2873
                     /label=T7 TE
     misc_feature    2880..2880
                     /label=stop
     polyA_site      2874..2887
                     /label=polya
     misc_feature    2847..2887
                     /label=BBa_B0012
     terminator      2847..2887
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggaaa gtactagatg gcttcctccg aagacgttat
     1261 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     1321 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     1381 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     1441 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     1501 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
     1561 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     1621 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     1681 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     1741 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     1801 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     1861 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
     1921 ctgatagtgc tagtgtagat cgctactaga ggaattaggt cttcgaatca caaagattag
     1981 tcacacgcga tccattgcag tggtactaga gtcacacagg aaagtactag atgcgtaaag
     2041 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     2101 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     2161 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     2221 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
     2281 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     2341 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     2401 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     2461 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     2521 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     2581 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     2641 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     2701 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
     2761 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2821 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2881 gtttata
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