LOCUS       BBa_I13513              2885 bp    DNA     linear       25-Nov-2024
DEFINITION  Screening Plasmid 2
ACCESSION   BBa_I13513
VERSION     BBa_I13513.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1911
                     /label=mrfp1
     misc_feature    1918..1942
                     /label=Help:Barcodes
     CDS             1237..1917
                     /label=BBa_E1010
     misc_feature    1951..1956
                     /label=Future Eco site
     misc_feature    1958..1963
                     /label=BbsI site
     misc_feature    1991..2002
                     /label=BstXI site
     misc_feature    1994..1999
                     /label=Future Pst site
     misc_feature    1951..2002
                     /label=BBa_I13452
     misc_feature    2015..2018
                     /label=conserved
     RBS             2011..2022
                     /label=BBa_B0034
     CDS             2029..2748
                     /label=GFP protein
     CDS             2029..2748
                     /label=BBa_E0040
     stem_loop       2768..2811
                     /label=stem_loop
     misc_feature    2757..2836
                     /label=BBa_B0010
     terminator      2757..2836
                     /label=BBa_B0010
     stem_loop       2852..2871
                     /label=T7 TE
     misc_feature    2878..2878
                     /label=stop
     polyA_site      2872..2885
                     /label=polya
     misc_feature    2845..2885
                     /label=BBa_B0012
     terminator      2845..2885
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1921 tgatagtgct agtgtagatc gctactagag gaattaggtc ttcgaatcac aaagattagt
     1981 cacacgcgat ccattgcagt ggtactagag aaagaggaga aatactagat gcgtaaagga
     2041 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     2101 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     2161 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     2221 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     2281 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     2341 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     2401 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     2461 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     2521 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     2581 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     2641 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     2701 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
     2761 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2821 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2881 ttata
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