LOCUS       BBa_I13519               936 bp    DNA     linear       22-Dec-2024
DEFINITION  Screening Plasmid Intermediate
ACCESSION   BBa_I13519
VERSION     BBa_I13519.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=Future Eco site
     misc_feature    8..13
                     /label=BbsI site
     misc_feature    41..52
                     /label=BstXI site
     misc_feature    44..49
                     /label=Future Pst site
     misc_feature    1..52
                     /label=BBa_I13452
     RBS             61..73
                     /label=RBS-3\Weak
     RBS             67..70
                     /label=RBS
     misc_feature    61..73
                     /label=BBa_B0032
     RBS             61..73
                     /label=BBa_B0032
     CDS             80..799
                     /label=GFP protein
     CDS             80..799
                     /label=BBa_E0040
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
ORIGIN
        1 gaattaggtc ttcgaatcac aaagattagt cacacgcgat ccattgcagt ggtactagag
       61 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      121 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      181 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      241 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      301 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      361 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      421 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      481 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      541 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      601 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      661 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      721 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      781 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttata
//