LOCUS       BBa_I13523               961 bp    DNA     linear       22-Dec-2024
DEFINITION  Screening plasmid v2
ACCESSION   BBa_I13523
VERSION     BBa_I13523.1  
FEATURES             Location/Qualifiers
     misc_feature    1..44
                     /label=RNAse E site
     misc_feature    29..30
                     /label=RNAse E cut site (between the bases)
     misc_feature    45..78
                     /label=Insertion site
     misc_feature    1..78
                     /label=BBa_I13455
     misc_feature    91..94
                     /label=conserved
     RBS             87..98
                     /label=BBa_B0034
     CDS             105..824
                     /label=GFP protein
     CDS             105..824
                     /label=BBa_E0040
     stem_loop       844..887
                     /label=stem_loop
     misc_feature    833..912
                     /label=BBa_B0010
     terminator      833..912
                     /label=BBa_B0010
     stem_loop       928..947
                     /label=T7 TE
     misc_feature    954..954
                     /label=stop
     polyA_site      948..961
                     /label=polya
     misc_feature    921..961
                     /label=BBa_B0012
     terminator      921..961
                     /label=BBa_B0012
ORIGIN
        1 ccgcggtgaa aatacgagaa tattatttgt attgatctcc tagggaatta ggtcttcgaa
       61 tccgatccat tgcagtggta ctagagaaag aggagaaata ctagatgcgt aaaggagaag
      121 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      181 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      241 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
      301 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      361 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      421 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      481 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      541 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      601 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      661 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      721 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      781 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
      841 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      901 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      961 a
//