LOCUS       BBa_I13524               947 bp    DNA     linear       22-Dec-2024
DEFINITION  Screening plasmid v2 intermediate
ACCESSION   BBa_I13524
VERSION     BBa_I13524.1  
FEATURES             Location/Qualifiers
     misc_feature    1..44
                     /label=RNAse E site
     misc_feature    29..30
                     /label=RNAse E cut site (between the bases)
     misc_feature    45..78
                     /label=Insertion site
     misc_feature    1..78
                     /label=BBa_I13455
     misc_feature    91..94
                     /label=conserved
     RBS             87..98
                     /label=BBa_B0034
     CDS             105..779
                     /label=mrfp1
     misc_feature    786..810
                     /label=Help:Barcodes
     CDS             105..785
                     /label=BBa_E1010
     stem_loop       830..873
                     /label=stem_loop
     misc_feature    819..898
                     /label=BBa_B0010
     terminator      819..898
                     /label=BBa_B0010
     stem_loop       914..933
                     /label=T7 TE
     misc_feature    940..940
                     /label=stop
     polyA_site      934..947
                     /label=polya
     misc_feature    907..947
                     /label=BBa_B0012
     terminator      907..947
                     /label=BBa_B0012
ORIGIN
        1 ccgcggtgaa aatacgagaa tattatttgt attgatctcc tagggaatta ggtcttcgaa
       61 tccgatccat tgcagtggta ctagagaaag aggagaaata ctagatggct tcctccgaag
      121 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
      181 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
      241 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
      301 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
      361 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
      421 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
      481 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
      541 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
      601 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
      661 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
      721 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      781 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
      841 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      901 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//