LOCUS       BBa_I13528               980 bp    DNA     linear       20-Apr-2024
DEFINITION  Screening plasmid v1.0 intermediate
ACCESSION   BBa_I13528
VERSION     BBa_I13528.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=RNAse E site
     misc_feature    9..10
                     /label=RNAse E cut site (between the bases)
     misc_feature    22..55
                     /label=Insertion site
     misc_feature    56..76
                     /label=RNAse E site
     misc_feature    64..65
                     /label=RNAse E cut site (between the bases)
     stem_loop       77..111
                     /label=Hairpin
     misc_feature    1..111
                     /label=BBa_I13457
     misc_feature    124..127
                     /label=conserved
     RBS             120..131
                     /label=BBa_B0034
     CDS             138..812
                     /label=mrfp1
     misc_feature    819..843
                     /label=Help:Barcodes
     CDS             138..818
                     /label=BBa_E1010
     stem_loop       863..906
                     /label=stem_loop
     misc_feature    852..931
                     /label=BBa_B0010
     terminator      852..931
                     /label=BBa_B0010
     stem_loop       947..966
                     /label=T7 TE
     misc_feature    973..973
                     /label=stop
     polyA_site      967..980
                     /label=polya
     misc_feature    940..980
                     /label=BBa_B0012
     terminator      940..980
                     /label=BBa_B0012
     misc_feature    120..980
                     /label=BBa_I13507
ORIGIN
        1 tattatttgt attgatctgg tgaattaggt cttcgaatcc gatccattgc agtggtatta
       61 tttgtattga tctccttact atctctcgag agattagtac ctttggagat ctactagaga
      121 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca
      181 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag
      241 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc
      301 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac
      361 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac
      421 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag
      481 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg
      541 ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg
      601 gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg
      661 ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa
      721 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg
      781 aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat
      841 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      961 tgggcctttc tgcgtttata
//