LOCUS       BBa_I13529              2911 bp    DNA     linear       12-May-2024
DEFINITION  Screening Plasmid 2.1
ACCESSION   BBa_I13529
VERSION     BBa_I13529.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1911
                     /label=mrfp1
     misc_feature    1918..1942
                     /label=Help:Barcodes
     CDS             1237..1917
                     /label=BBa_E1010
     misc_feature    1951..1994
                     /label=RNAse E site
     misc_feature    1979..1980
                     /label=RNAse E cut site (between the bases)
     misc_feature    1995..2028
                     /label=Insertion site
     misc_feature    1951..2028
                     /label=BBa_I13455
     misc_feature    2041..2044
                     /label=conserved
     RBS             2037..2048
                     /label=BBa_B0034
     CDS             2055..2774
                     /label=GFP protein
     CDS             2055..2774
                     /label=BBa_E0040
     stem_loop       2794..2837
                     /label=stem_loop
     misc_feature    2783..2862
                     /label=BBa_B0010
     terminator      2783..2862
                     /label=BBa_B0010
     stem_loop       2878..2897
                     /label=T7 TE
     misc_feature    2904..2904
                     /label=stop
     polyA_site      2898..2911
                     /label=polya
     misc_feature    2871..2911
                     /label=BBa_B0012
     terminator      2871..2911
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1921 tgatagtgct agtgtagatc gctactagag ccgcggtgaa aatacgagaa tattatttgt
     1981 attgatctcc tagggaatta ggtcttcgaa tccgatccat tgcagtggta ctagagaaag
     2041 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     2101 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     2161 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2221 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
     2281 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     2341 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     2401 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     2461 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     2521 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
     2581 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     2641 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     2701 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     2761 tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     2821 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     2881 tcaccttcgg gtgggccttt ctgcgtttat a
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