LOCUS       BBa_I13533              2944 bp    DNA     linear       22-Nov-2024
DEFINITION  Screening Plasmid 2.5
ACCESSION   BBa_I13533
VERSION     BBa_I13533.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1911
                     /label=mrfp1
     misc_feature    1918..1942
                     /label=Help:Barcodes
     CDS             1237..1917
                     /label=BBa_E1010
     misc_feature    1..1942
                     /label=BBa_I13516
     misc_feature    1951..1971
                     /label=RNAse E site
     misc_feature    1959..1960
                     /label=RNAse E cut site (between the bases)
     misc_feature    1972..2005
                     /label=Insertion site
     misc_feature    2006..2026
                     /label=RNAse E site
     misc_feature    2014..2015
                     /label=RNAse E cut site (between the bases)
     stem_loop       2027..2061
                     /label=Hairpin
     misc_feature    1951..2061
                     /label=BBa_I13457
     misc_feature    2074..2077
                     /label=conserved
     RBS             2070..2081
                     /label=BBa_B0034
     CDS             2088..2807
                     /label=GFP protein
     CDS             2088..2807
                     /label=BBa_E0040
     stem_loop       2827..2870
                     /label=stem_loop
     misc_feature    2816..2895
                     /label=BBa_B0010
     terminator      2816..2895
                     /label=BBa_B0010
     stem_loop       2911..2930
                     /label=T7 TE
     misc_feature    2937..2937
                     /label=stop
     polyA_site      2931..2944
                     /label=polya
     misc_feature    2904..2944
                     /label=BBa_B0012
     terminator      2904..2944
                     /label=BBa_B0012
     misc_feature    2070..2944
                     /label=BBa_I13504
     misc_feature    1951..2944
                     /label=BBa_I13527
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1921 tgatagtgct agtgtagatc gctactagag tattatttgt attgatctgg tgaattaggt
     1981 cttcgaatcc gatccattgc agtggtatta tttgtattga tctccttact atctctcgag
     2041 agattagtac ctttggagat ctactagaga aagaggagaa atactagatg cgtaaaggag
     2101 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     2161 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     2221 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
     2281 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
     2341 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     2401 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     2461 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     2521 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     2581 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     2641 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     2701 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     2761 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
     2821 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2881 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2941 tata
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