##gff-version 3
##sequence-region BBa_I13533 1 2944
BBa_I13533	.	engineered_region	1	1942	.	+	0	ID=BBa_I13516;Name=BBa_I13516
BBa_I13533	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_I13516
BBa_I13533	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I13533	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I13533	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I13533	.	CDS	1237	1917	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13516
BBa_I13533	.	CDS	1237	1911	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13533	.	engineered_tag	1918	1942	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13533	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13516
BBa_I13533	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13533	.	engineered_region	1951	2944	.	+	0	ID=BBa_I13527;Name=BBa_I13527
BBa_I13533	.	engineered_region	2070	2944	.	+	0	ID=BBa_I13504;Name=BBa_I13504;Parent=BBa_I13527
BBa_I13533	.	ribosome_entry_site	2070	2081	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_I13533	.	conserved_region	2074	2077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13533	.	CDS	2088	2807	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_I13533	.	CDS	2088	2807	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I13533	.	terminator	2904	2944	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_I13533	.	stem_loop	2911	2930	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13533	.	polyA_site	2931	2944	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13533	.	stop_codon	2937	2937	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13533	.	engineered_region	2904	2944	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13533	.	terminator	2816	2895	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_I13533	.	stem_loop	2827	2870	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13533	.	engineered_region	2816	2895	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13533	.	sequence_feature	1951	2061	.	+	0	ID=BBa_I13457;Name=BBa_I13457;Parent=BBa_I13527
BBa_I13533	.	sequence_feature	2014	2015	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13533	.	sequence_feature	1959	1960	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13533	.	stem_loop	2027	2061	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13533	.	sequence_feature	2006	2026	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
BBa_I13533	.	sequence_feature	1972	2005	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
BBa_I13533	.	sequence_feature	1951	1971	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
>BBa_I13533
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaag
agttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcg
tccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcc
ccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccgg
aaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaaga
cggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatg
ggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactga
aagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa
aaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgt
cactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtattatttgtattgatctggtgaat
taggtcttcgaatccgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattagtaccttt
ggagatctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttg
ttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaact
tacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgtt
caatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacagg
aaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaa
tagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactca
cacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatg
gaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccatta
cctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagct
gctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata