LOCUS       BBa_I13534              2944 bp    DNA     linear       22-Dec-2024
DEFINITION  Screening Plasmid 2.6
ACCESSION   BBa_I13534
VERSION     BBa_I13534.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1956
                     /label=GFP protein
     CDS             1237..1956
                     /label=BBa_E0040
     misc_feature    1..1956
                     /label=BBa_I13506
     misc_feature    1965..1985
                     /label=RNAse E site
     misc_feature    1973..1974
                     /label=RNAse E cut site (between the bases)
     misc_feature    1986..2019
                     /label=Insertion site
     misc_feature    2020..2040
                     /label=RNAse E site
     misc_feature    2028..2029
                     /label=RNAse E cut site (between the bases)
     stem_loop       2041..2075
                     /label=Hairpin
     misc_feature    1965..2075
                     /label=BBa_I13457
     misc_feature    2088..2091
                     /label=conserved
     RBS             2084..2095
                     /label=BBa_B0034
     CDS             2102..2776
                     /label=mrfp1
     misc_feature    2783..2807
                     /label=Help:Barcodes
     CDS             2102..2782
                     /label=BBa_E1010
     stem_loop       2827..2870
                     /label=stem_loop
     misc_feature    2816..2895
                     /label=BBa_B0010
     terminator      2816..2895
                     /label=BBa_B0010
     stem_loop       2911..2930
                     /label=T7 TE
     misc_feature    2937..2937
                     /label=stop
     polyA_site      2931..2944
                     /label=polya
     misc_feature    2904..2944
                     /label=BBa_B0012
     terminator      2904..2944
                     /label=BBa_B0012
     misc_feature    2084..2944
                     /label=BBa_I13507
     misc_feature    1965..2944
                     /label=BBa_I13528
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1921 attacacatg gcatggatga actatacaaa taataatact agagtattat ttgtattgat
     1981 ctggtgaatt aggtcttcga atccgatcca ttgcagtggt attatttgta ttgatctcct
     2041 tactatctct cgagagatta gtacctttgg agatctacta gagaaagagg agaaatacta
     2101 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     2161 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     2221 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     2281 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     2341 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     2401 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     2461 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     2521 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
     2581 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     2641 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     2701 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     2761 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
     2821 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2881 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2941 tata
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