BBa_I13537
type: ComponentDefinition
persistent identity: BBa I13537
display id: BBa_I13537
version: 1
was generated by: iGEM to SBOL conversion
description: Two promoter screening plasmid v1.0
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_E0040
component: BBa_B0034
component: BBa_I13453
component: BBa_B0012
component: BBa_B0010
component: BBa_E1010
component: BBa_I13457
component: BBa_I13458
sequence annotation: BBa_I13458
sequence annotation: BBa_I13453
sequence annotation: BBa_I13457
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13537_sequence/1

BBa_E0040
type: Component
persistent identity: component1663271
display id: component1663271
version: 1
top level: BBa_I13537
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1663269
display id: component1663269
version: 1
top level: BBa_I13537
definition: BBa_B0034
access: public

BBa_I13453
type: Component
persistent identity: component1663264
display id: component1663264
version: 1
top level: BBa_I13537
definition: BBa_I13453
access: public

BBa_B0012
type: Component
persistent identity: component1663259
display id: component1663259
version: 1
top level: BBa_I13537
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1663249
display id: component1663249
version: 1
top level: BBa_I13537
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1663243
display id: component1663243
version: 1
top level: BBa_I13537
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component1663236
display id: component1663236
version: 1
top level: BBa_I13537
definition: BBa_B0034
access: public

BBa_I13457
type: Component
persistent identity: component1663231
display id: component1663231
version: 1
top level: BBa_I13537
definition: BBa_I13457
access: public

BBa_I13453
type: Component
persistent identity: component1663211
display id: component1663211
version: 1
top level: BBa_I13537
definition: BBa_I13453
access: public

BBa_I13458
type: Component
persistent identity: component1663209
display id: component1663209
version: 1
top level: BBa_I13537
definition: BBa_I13458
access: public

BBa_B0012
type: Component
persistent identity: component1663286
display id: component1663286
version: 1
top level: BBa_I13537
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1663276
display id: component1663276
version: 1
top level: BBa_I13537
definition: BBa_B0010
access: public

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation1663209
display id: annotation1663209
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663209/range1663209/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_I13537/annotation1663209/range1663209/1
type: Range
persistent identity: range1663209
display id: range1663209
version: 1
top level: BBa_I13537
direction: direction/forward
start: 1
end: 1114
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663211
display id: annotation1663211
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663211/range1663211/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13537/annotation1663211/range1663211/1
type: Range
persistent identity: range1663211
display id: range1663211
version: 1
top level: BBa_I13537
direction: direction/forward
start: 1123
end: 1252
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1663231
display id: annotation1663231
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663231/range1663231/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13537/annotation1663231/range1663231/1
type: Range
persistent identity: range1663231
display id: range1663231
version: 1
top level: BBa_I13537
direction: direction/forward
start: 1261
end: 1371
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663236
display id: annotation1663236
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663236/range1663236/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13537/annotation1663236/range1663236/1
type: Range
persistent identity: range1663236
display id: range1663236
version: 1
top level: BBa_I13537
direction: direction/forward
start: 1380
end: 1391
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1663243
display id: annotation1663243
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663243/range1663243/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13537/annotation1663243/range1663243/1
type: Range
persistent identity: range1663243
display id: range1663243
version: 1
top level: BBa_I13537
direction: direction/forward
start: 1398
end: 2078
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663249
display id: annotation1663249
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663249/range1663249/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13537/annotation1663249/range1663249/1
type: Range
persistent identity: range1663249
display id: range1663249
version: 1
top level: BBa_I13537
direction: direction/forward
start: 2112
end: 2191
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663259
display id: annotation1663259
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663259/range1663259/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13537/annotation1663259/range1663259/1
type: Range
persistent identity: range1663259
display id: range1663259
version: 1
top level: BBa_I13537
direction: direction/forward
start: 2200
end: 2240
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663264
display id: annotation1663264
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663264/range1663264/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13537/annotation1663264/range1663264/1
type: Range
persistent identity: range1663264
display id: range1663264
version: 1
top level: BBa_I13537
direction: direction/forward
start: 2249
end: 2378
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663269
display id: annotation1663269
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663269/range1663269/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13537/annotation1663269/range1663269/1
type: Range
persistent identity: range1663269
display id: range1663269
version: 1
top level: BBa_I13537
direction: direction/forward
start: 2387
end: 2398
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1663271
display id: annotation1663271
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663271/range1663271/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13537/annotation1663271/range1663271/1
type: Range
persistent identity: range1663271
display id: range1663271
version: 1
top level: BBa_I13537
direction: direction/forward
start: 2405
end: 3124
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663276
display id: annotation1663276
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663276/range1663276/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13537/annotation1663276/range1663276/1
type: Range
persistent identity: range1663276
display id: range1663276
version: 1
top level: BBa_I13537
direction: direction/forward
start: 3133
end: 3212
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663286
display id: annotation1663286
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663286/range1663286/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13537/annotation1663286/range1663286/1
type: Range
persistent identity: range1663286
display id: range1663286
version: 1
top level: BBa_I13537
direction: direction/forward
start: 3221
end: 3261
orientation: inline

BBa_I13457
type: ComponentDefinition
persistent identity: BBa I13457
display id: BBa_I13457
version: 1
was generated by: iGEM to SBOL conversion
description: Improved insertion site with two RNAse E cut sites
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Shortened RNAse E site, insertion site, duplicate RNAse E site, and a trailing 5' hairpin (HP16 from Smolke et al., 2002).
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: RNAse E site
sequence annotation: RNAse E cut site (between the bases)
sequence annotation: Insertion site
sequence annotation: Hairpin
sequence: https://synbiohub.org/public/igem/BBa_I13457_sequence/1

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588510
display id: annotation1588510
version: 1
top level: BBa_I13457
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588510/range1588510/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I13457/annotation1588510/range1588510/1
type: Range
persistent identity: range1588510
display id: range1588510
version: 1
top level: BBa_I13457
direction: direction/forward
start: 1
end: 21
orientation: inline

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588511
display id: annotation1588511
version: 1
top level: BBa_I13457
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588511/range1588511/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I13457/annotation1588511/range1588511/1
type: Range
persistent identity: range1588511
display id: range1588511
version: 1
top level: BBa_I13457
direction: direction/forward
start: 9
end: 10
orientation: inline

Insertion site
type: SequenceAnnotation
persistent identity: annotation1588512
display id: annotation1588512
version: 1
top level: BBa_I13457
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588512/range1588512/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I13457/annotation1588512/range1588512/1
type: Range
persistent identity: range1588512
display id: range1588512
version: 1
top level: BBa_I13457
direction: direction/forward
start: 22
end: 55
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1588513
display id: annotation1588513
version: 1
top level: BBa_I13457
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588513/range1588513/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I13457/annotation1588513/range1588513/1
type: Range
persistent identity: range1588513
display id: range1588513
version: 1
top level: BBa_I13457
direction: direction/forward
start: 56
end: 76
orientation: inline

RNAse E cut site (between the bases)
type: SequenceAnnotation
persistent identity: annotation1588514
display id: annotation1588514
version: 1
top level: BBa_I13457
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588514/range1588514/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I13457/annotation1588514/range1588514/1
type: Range
persistent identity: range1588514
display id: range1588514
version: 1
top level: BBa_I13457
direction: direction/forward
start: 64
end: 65
orientation: inline

Hairpin
type: SequenceAnnotation
persistent identity: annotation1588515
display id: annotation1588515
version: 1
top level: BBa_I13457
location: https://synbiohub.org/public/igem/BBa_I13457/annotation1588515/range1588515/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I13457/annotation1588515/range1588515/1
type: Range
persistent identity: range1588515
display id: range1588515
version: 1
top level: BBa_I13457
direction: direction/forward
start: 77
end: 111
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_I13458
type: ComponentDefinition
persistent identity: BBa I13458
display id: BBa_I13458
version: 1
was generated by: iGEM to SBOL conversion
description: promoter C and arabinose C operon
created: 2005-08-16T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I13458_sequence/1

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_I13537_sequence/1
type: Sequence
persistent identity: BBa I13537 sequence
display id: BBa_I13537_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtattatttgtattgatctggtgaattaggtcttcgaatccgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattagtacctttggagatctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13457_sequence/1
type: Sequence
persistent identity: BBa I13457 sequence
display id: BBa_I13457_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tattatttgtattgatctggtgaattaggtcttcgaatccgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattagtacctttggagatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13458_sequence/1
type: Sequence
persistent identity: BBa I13458 sequence
display id: BBa_I13458_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z