LOCUS       BBa_I13538              3219 bp    DNA     linear       26-Dec-2024
DEFINITION  Two promoter screening plasmid v1.1
ACCESSION   BBa_I13538
VERSION     BBa_I13538.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1956
                     /label=GFP protein
     CDS             1237..1956
                     /label=BBa_E0040
     stem_loop       1976..2019
                     /label=stem_loop
     misc_feature    1965..2044
                     /label=BBa_B0010
     terminator      1965..2044
                     /label=BBa_B0010
     stem_loop       2060..2079
                     /label=T7 TE
     misc_feature    2086..2086
                     /label=stop
     polyA_site      2080..2093
                     /label=polya
     misc_feature    2053..2093
                     /label=BBa_B0012
     terminator      2053..2093
                     /label=BBa_B0012
     promoter        2102..2231
                     /label=BBa_I13453
     misc_feature    2240..2260
                     /label=RNAse E site
     misc_feature    2248..2249
                     /label=RNAse E cut site (between the bases)
     misc_feature    2261..2294
                     /label=Insertion site
     misc_feature    2295..2315
                     /label=RNAse E site
     misc_feature    2303..2304
                     /label=RNAse E cut site (between the bases)
     stem_loop       2316..2350
                     /label=Hairpin
     misc_feature    2240..2350
                     /label=BBa_I13457
     misc_feature    2363..2366
                     /label=conserved
     RBS             2359..2370
                     /label=BBa_B0034
     CDS             2377..3051
                     /label=mrfp1
     misc_feature    3058..3082
                     /label=Help:Barcodes
     CDS             2377..3057
                     /label=BBa_E1010
     stem_loop       3102..3145
                     /label=stem_loop
     misc_feature    3091..3170
                     /label=BBa_B0010
     terminator      3091..3170
                     /label=BBa_B0010
     stem_loop       3186..3205
                     /label=T7 TE
     misc_feature    3212..3212
                     /label=stop
     polyA_site      3206..3219
                     /label=polya
     misc_feature    3179..3219
                     /label=BBa_B0012
     terminator      3179..3219
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1921 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
     1981 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2041 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     2101 gacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
     2161 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc ataccgtttt
     2221 tttgggctag ctactagagt attatttgta ttgatctggt gaattaggtc ttcgaatccg
     2281 atccattgca gtggtattat ttgtattgat ctccttacta tctctcgaga gattagtacc
     2341 tttggagatc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     2401 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     2461 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     2521 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     2581 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     2641 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     2701 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     2761 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     2821 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     2881 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     2941 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     3001 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     3061 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt
     3121 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     3181 acactggctc accttcgggt gggcctttct gcgtttata
//