LOCUS BBa_I13538 3219 bp DNA linear 08-May-2024 DEFINITION Two promoter screening plasmid v1.1 ACCESSION BBa_I13538 VERSION BBa_I13538.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1956 /label=GFP protein CDS 1237..1956 /label=BBa_E0040 stem_loop 1976..2019 /label=stem_loop misc_feature 1965..2044 /label=BBa_B0010 terminator 1965..2044 /label=BBa_B0010 stem_loop 2060..2079 /label=T7 TE misc_feature 2086..2086 /label=stop polyA_site 2080..2093 /label=polya misc_feature 2053..2093 /label=BBa_B0012 terminator 2053..2093 /label=BBa_B0012 promoter 2102..2231 /label=BBa_I13453 misc_feature 2240..2260 /label=RNAse E site misc_feature 2248..2249 /label=RNAse E cut site (between the bases) misc_feature 2261..2294 /label=Insertion site misc_feature 2295..2315 /label=RNAse E site misc_feature 2303..2304 /label=RNAse E cut site (between the bases) stem_loop 2316..2350 /label=Hairpin misc_feature 2240..2350 /label=BBa_I13457 misc_feature 2363..2366 /label=conserved RBS 2359..2370 /label=BBa_B0034 CDS 2377..3051 /label=mrfp1 misc_feature 3058..3082 /label=Help:Barcodes CDS 2377..3057 /label=BBa_E1010 stem_loop 3102..3145 /label=stem_loop misc_feature 3091..3170 /label=BBa_B0010 terminator 3091..3170 /label=BBa_B0010 stem_loop 3186..3205 /label=T7 TE misc_feature 3212..3212 /label=stop polyA_site 3206..3219 /label=polya misc_feature 3179..3219 /label=BBa_B0012 terminator 3179..3219 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc 1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt 1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 1921 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa 1981 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 2041 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 2101 gacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga 2161 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc ataccgtttt 2221 tttgggctag ctactagagt attatttgta ttgatctggt gaattaggtc ttcgaatccg 2281 atccattgca gtggtattat ttgtattgat ctccttacta tctctcgaga gattagtacc 2341 tttggagatc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc 2401 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 2461 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 2521 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 2581 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 2641 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc 2701 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 2761 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 2821 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa 2881 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag 2941 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac 3001 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc 3061 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 3121 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3181 acactggctc accttcgggt gggcctttct gcgtttata //