LOCUS       BBa_I13539              3219 bp    DNA     linear       22-Nov-2024
DEFINITION  Two promoter screening plasmid v1.2
ACCESSION   BBa_I13539
VERSION     BBa_I13539.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1219..1239
                     /label=RNAse E site
     misc_feature    1227..1228
                     /label=RNAse E cut site (between the bases)
     misc_feature    1240..1273
                     /label=Insertion site
     misc_feature    1274..1294
                     /label=RNAse E site
     misc_feature    1282..1283
                     /label=RNAse E cut site (between the bases)
     stem_loop       1295..1329
                     /label=Hairpin
     misc_feature    1219..1329
                     /label=BBa_I13457
     misc_feature    1342..1345
                     /label=conserved
     RBS             1338..1349
                     /label=BBa_B0034
     CDS             1356..2030
                     /label=mrfp1
     misc_feature    2037..2061
                     /label=Help:Barcodes
     CDS             1356..2036
                     /label=BBa_E1010
     stem_loop       2081..2124
                     /label=stem_loop
     misc_feature    2070..2149
                     /label=BBa_B0010
     terminator      2070..2149
                     /label=BBa_B0010
     stem_loop       2165..2184
                     /label=T7 TE
     misc_feature    2191..2191
                     /label=stop
     polyA_site      2185..2198
                     /label=polya
     misc_feature    2158..2198
                     /label=BBa_B0012
     terminator      2158..2198
                     /label=BBa_B0012
     promoter        2207..2336
                     /label=BBa_I13453
     misc_feature    2349..2352
                     /label=conserved
     RBS             2345..2356
                     /label=BBa_B0034
     CDS             2363..3082
                     /label=GFP protein
     CDS             2363..3082
                     /label=BBa_E0040
     stem_loop       3102..3145
                     /label=stem_loop
     misc_feature    3091..3170
                     /label=BBa_B0010
     terminator      3091..3170
                     /label=BBa_B0010
     stem_loop       3186..3205
                     /label=T7 TE
     misc_feature    3212..3212
                     /label=stop
     polyA_site      3206..3219
                     /label=polya
     misc_feature    3179..3219
                     /label=BBa_B0012
     terminator      3179..3219
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagta ttatttgtat tgatctggtg aattaggtct tcgaatccga
     1261 tccattgcag tggtattatt tgtattgatc tccttactat ctctcgagag attagtacct
     1321 ttggagatct actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
     1381 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
     1441 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
     1501 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
     1561 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
     1621 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
     1681 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
     1741 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
     1801 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
     1861 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     1921 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
     1981 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
     2041 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     2101 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2161 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacat tgattatttg
     2221 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc
     2281 tgacgctttt tatcgcaact ctctactgtt tctccatacc gtttttttgg gctagctact
     2341 agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
     2401 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     2461 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     2521 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
     2581 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
     2641 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     2701 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     2761 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     2821 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     2881 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     2941 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     3001 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     3061 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     3121 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     3181 acactggctc accttcgggt gggcctttct gcgtttata
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