LOCUS       BBa_I13545              1726 bp    DNA     linear       10-May-2024
DEFINITION  GFP and RFP polycistronic
ACCESSION   BBa_I13545
VERSION     BBa_I13545.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     misc_feature    1..738
                     /label=BBa_I13500
     misc_feature    747..767
                     /label=RNAse E site
     misc_feature    755..756
                     /label=RNAse E cut site (between the bases)
     misc_feature    768..801
                     /label=Insertion site
     misc_feature    802..822
                     /label=RNAse E site
     misc_feature    810..811
                     /label=RNAse E cut site (between the bases)
     stem_loop       823..857
                     /label=Hairpin
     misc_feature    747..857
                     /label=BBa_I13457
     misc_feature    870..873
                     /label=conserved
     RBS             866..877
                     /label=BBa_B0034
     CDS             884..1558
                     /label=mrfp1
     misc_feature    1565..1589
                     /label=Help:Barcodes
     CDS             884..1564
                     /label=BBa_E1010
     stem_loop       1609..1652
                     /label=stem_loop
     misc_feature    1598..1677
                     /label=BBa_B0010
     terminator      1598..1677
                     /label=BBa_B0010
     stem_loop       1693..1712
                     /label=T7 TE
     misc_feature    1719..1719
                     /label=stop
     polyA_site      1713..1726
                     /label=polya
     misc_feature    1686..1726
                     /label=BBa_B0012
     terminator      1686..1726
                     /label=BBa_B0012
     misc_feature    866..1726
                     /label=BBa_I13507
     misc_feature    747..1726
                     /label=BBa_I13528
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagtatt atttgtattg atctggtgaa ttaggtcttc
      781 gaatccgatc cattgcagtg gtattatttg tattgatctc cttactatct ctcgagagat
      841 tagtaccttt ggagatctac tagagaaaga ggagaaatac tagatggctt cctccgaaga
      901 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
      961 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
     1021 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
     1081 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
     1141 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
     1201 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
     1261 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
     1321 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
     1381 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
     1441 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
     1501 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
     1561 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
     1621 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1681 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//