##gff-version 3
##sequence-region BBa_I13545 1 1726
BBa_I13545	.	engineered_region	1	738	.	+	0	ID=BBa_I13500;Name=BBa_I13500
BBa_I13545	.	CDS	19	738	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13500
BBa_I13545	.	CDS	19	738	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I13545	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13500
BBa_I13545	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13545	.	engineered_region	747	1726	.	+	0	ID=BBa_I13528;Name=BBa_I13528
BBa_I13545	.	sequence_feature	747	857	.	+	0	ID=BBa_I13457;Name=BBa_I13457;Parent=BBa_I13528
BBa_I13545	.	sequence_feature	747	767	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
BBa_I13545	.	sequence_feature	802	822	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
BBa_I13545	.	sequence_feature	768	801	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
BBa_I13545	.	stem_loop	823	857	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13545	.	sequence_feature	755	756	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13545	.	sequence_feature	810	811	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13545	.	engineered_region	866	1726	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_I13528
BBa_I13545	.	terminator	1598	1677	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_I13545	.	stem_loop	1609	1652	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13545	.	engineered_region	1598	1677	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13545	.	terminator	1686	1726	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_I13545	.	stem_loop	1693	1712	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13545	.	stop_codon	1719	1719	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13545	.	engineered_region	1686	1726	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13545	.	polyA_site	1713	1726	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13545	.	CDS	884	1564	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_I13545	.	engineered_tag	1565	1589	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13545	.	CDS	884	1558	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13545	.	ribosome_entry_site	866	877	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_I13545	.	conserved_region	870	873	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13545
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaataataatactagagtattatttgtattgatctggtgaattaggtcttcgaatccgatc
cattgcagtggtattatttgtattgatctccttactatctctcgagagattagtacctttggagatctactagagaaag
aggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgt
taacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagtt
accaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaac
acccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaaga
cggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc
aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaag
acggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccac
ctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaa
gactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagt
gtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata