LOCUS BBa_I13546 2795 bp DNA linear 22-Nov-2024 DEFINITION AHL-induced GFP and RFP polycistronic reporter ACCESSION BBa_I13546 VERSION BBa_I13546.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..1807 /label=GFP protein CDS 1088..1807 /label=BBa_E0040 misc_feature 1070..1807 /label=BBa_I13500 misc_feature 1816..1836 /label=RNAse E site misc_feature 1824..1825 /label=RNAse E cut site (between the bases) misc_feature 1837..1870 /label=Insertion site misc_feature 1871..1891 /label=RNAse E site misc_feature 1879..1880 /label=RNAse E cut site (between the bases) stem_loop 1892..1926 /label=Hairpin misc_feature 1816..1926 /label=BBa_I13457 misc_feature 1939..1942 /label=conserved RBS 1935..1946 /label=BBa_B0034 CDS 1953..2627 /label=mrfp1 misc_feature 2634..2658 /label=Help:Barcodes CDS 1953..2633 /label=BBa_E1010 stem_loop 2678..2721 /label=stem_loop misc_feature 2667..2746 /label=BBa_B0010 terminator 2667..2746 /label=BBa_B0010 stem_loop 2762..2781 /label=T7 TE misc_feature 2788..2788 /label=stop polyA_site 2782..2795 /label=polya misc_feature 2755..2795 /label=BBa_B0012 terminator 2755..2795 /label=BBa_B0012 misc_feature 1935..2795 /label=BBa_I13507 misc_feature 1816..2795 /label=BBa_I13528 misc_feature 1070..2795 /label=BBa_I13545 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt 1141 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac 1201 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc 1261 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat 1321 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat 1381 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac 1441 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg 1501 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa 1561 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact 1621 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 1681 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat 1741 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa 1801 ataataatac tagagtatta tttgtattga tctggtgaat taggtcttcg aatccgatcc 1861 attgcagtgg tattatttgt attgatctcc ttactatctc tcgagagatt agtacctttg 1921 gagatctact agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag 1981 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg 2041 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca 2101 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca 2161 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag 2221 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg 2281 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc 2341 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta 2401 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg 2461 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc 2521 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca 2581 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat 2641 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2701 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2761 tggctcacct tcgggtgggc ctttctgcgt ttata //