##gff-version 3
##sequence-region BBa_I13546 1 2795
BBa_I13546	.	engineered_region	1070	2795	.	+	0	ID=BBa_I13545;Name=BBa_I13545
BBa_I13546	.	engineered_region	1816	2795	.	+	0	ID=BBa_I13528;Name=BBa_I13528;Parent=BBa_I13545
BBa_I13546	.	sequence_feature	1816	1926	.	+	0	ID=BBa_I13457;Name=BBa_I13457;Parent=BBa_I13528
BBa_I13546	.	stem_loop	1892	1926	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13546	.	sequence_feature	1837	1870	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
BBa_I13546	.	sequence_feature	1824	1825	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13546	.	sequence_feature	1871	1891	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
BBa_I13546	.	sequence_feature	1816	1836	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
BBa_I13546	.	sequence_feature	1879	1880	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13546	.	engineered_region	1935	2795	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_I13528
BBa_I13546	.	terminator	2755	2795	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_I13546	.	polyA_site	2782	2795	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13546	.	stem_loop	2762	2781	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13546	.	engineered_region	2755	2795	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13546	.	stop_codon	2788	2788	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13546	.	CDS	1953	2633	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_I13546	.	engineered_tag	2634	2658	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13546	.	CDS	1953	2627	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13546	.	ribosome_entry_site	1935	1946	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_I13546	.	conserved_region	1939	1942	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13546	.	terminator	2667	2746	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_I13546	.	stem_loop	2678	2721	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13546	.	engineered_region	2667	2746	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13546	.	engineered_region	1070	1807	.	+	0	ID=BBa_I13500;Name=BBa_I13500;Parent=BBa_I13545
BBa_I13546	.	CDS	1088	1807	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13500
BBa_I13546	.	CDS	1088	1807	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I13546	.	ribosome_entry_site	1070	1081	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13500
BBa_I13546	.	conserved_region	1074	1077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13546	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_I13546	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_I13546	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I13546	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I13546	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I13546	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I13546	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I13546	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I13546	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_I13546	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I13546	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I13546	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I13546	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I13546	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I13546	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_I13546	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I13546	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I13546	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I13546	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I13546	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I13546	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_I13546	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I13546	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13546	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13546	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13546	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13546	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I13546	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13546	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13546	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_I13546	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I13546
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcgtaaaggagaagaac
ttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagaggg
tgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaaca
cttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaaga
gtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagt
caagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgga
cacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaact
tcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatgg
ccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccac
atggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagta
ttatttgtattgatctggtgaattaggtcttcgaatccgatccattgcagtggtattatttgtattgatctccttacta
tctctcgagagattagtacctttggagatctactagagaaagaggagaaatactagatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata