LOCUS BBa_I13547 2892 bp DNA linear 22-Nov-2024 DEFINITION AHL-induced GFP and RFP polycistronic reporter ACCESSION BBa_I13547 VERSION BBa_I13547.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 CDS 1185..1904 /label=GFP protein CDS 1185..1904 /label=BBa_E0040 misc_feature 1167..1904 /label=BBa_I13500 misc_feature 1913..1933 /label=RNAse E site misc_feature 1921..1922 /label=RNAse E cut site (between the bases) misc_feature 1934..1967 /label=Insertion site misc_feature 1968..1988 /label=RNAse E site misc_feature 1976..1977 /label=RNAse E cut site (between the bases) stem_loop 1989..2023 /label=Hairpin misc_feature 1913..2023 /label=BBa_I13457 misc_feature 2036..2039 /label=conserved RBS 2032..2043 /label=BBa_B0034 CDS 2050..2724 /label=mrfp1 misc_feature 2731..2755 /label=Help:Barcodes CDS 2050..2730 /label=BBa_E1010 stem_loop 2775..2818 /label=stem_loop misc_feature 2764..2843 /label=BBa_B0010 terminator 2764..2843 /label=BBa_B0010 stem_loop 2859..2878 /label=T7 TE misc_feature 2885..2885 /label=stop polyA_site 2879..2892 /label=polya misc_feature 2852..2892 /label=BBa_B0012 terminator 2852..2892 /label=BBa_B0012 misc_feature 2032..2892 /label=BBa_I13507 misc_feature 1913..2892 /label=BBa_I13528 misc_feature 1167..2892 /label=BBa_I13545 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgcgt aaaggagaag 1201 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca 1261 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat 1321 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt 1381 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga 1441 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact 1501 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa 1561 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata 1621 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca 1681 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata 1741 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg 1801 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 1861 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agtattattt 1921 gtattgatct ggtgaattag gtcttcgaat ccgatccatt gcagtggtat tatttgtatt 1981 gatctcctta ctatctctcg agagattagt acctttggag atctactaga gaaagaggag 2041 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt 2101 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg 2161 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct 2221 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct 2281 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg 2341 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag 2401 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag 2461 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg 2521 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt 2581 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc 2641 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct 2701 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta 2761 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 2821 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 2881 tctgcgttta ta //