LOCUS BBa_I13548 2796 bp DNA linear 25-Nov-2024 DEFINITION AHL-induced GFP and RFP polycistronic reporter ACCESSION BBa_I13548 VERSION BBa_I13548.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 CDS 1089..1808 /label=GFP protein CDS 1089..1808 /label=BBa_E0040 misc_feature 1071..1808 /label=BBa_I13500 misc_feature 1817..1837 /label=RNAse E site misc_feature 1825..1826 /label=RNAse E cut site (between the bases) misc_feature 1838..1871 /label=Insertion site misc_feature 1872..1892 /label=RNAse E site misc_feature 1880..1881 /label=RNAse E cut site (between the bases) stem_loop 1893..1927 /label=Hairpin misc_feature 1817..1927 /label=BBa_I13457 misc_feature 1940..1943 /label=conserved RBS 1936..1947 /label=BBa_B0034 CDS 1954..2628 /label=mrfp1 misc_feature 2635..2659 /label=Help:Barcodes CDS 1954..2634 /label=BBa_E1010 stem_loop 2679..2722 /label=stem_loop misc_feature 2668..2747 /label=BBa_B0010 terminator 2668..2747 /label=BBa_B0010 stem_loop 2763..2782 /label=T7 TE misc_feature 2789..2789 /label=stop polyA_site 2783..2796 /label=polya misc_feature 2756..2796 /label=BBa_B0012 terminator 2756..2796 /label=BBa_B0012 misc_feature 1936..2796 /label=BBa_I13507 misc_feature 1817..2796 /label=BBa_I13528 misc_feature 1071..2796 /label=BBa_I13545 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat 1141 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa 1201 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc 1261 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata 1321 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta 1381 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata 1441 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg 1501 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa 1561 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac 1621 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca 1681 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca 1741 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca 1801 aataataata ctagagtatt atttgtattg atctggtgaa ttaggtcttc gaatccgatc 1861 cattgcagtg gtattatttg tattgatctc cttactatct ctcgagagat tagtaccttt 1921 ggagatctac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa 1981 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc 2041 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc 2101 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc 2161 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa 2221 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag 2281 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc 2341 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt 2401 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac 2461 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg 2521 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc 2581 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga 2641 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2701 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2761 ctggctcacc ttcgggtggg cctttctgcg tttata //