LOCUS       BBa_I13550              2027 bp    DNA     linear       26-Dec-2024
DEFINITION  AHL-induced RFP reporter
ACCESSION   BBa_I13550
VERSION     BBa_I13550.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1171..1174
                     /label=conserved
     RBS             1167..1178
                     /label=BBa_B0034
     CDS             1185..1859
                     /label=mrfp1
     misc_feature    1866..1890
                     /label=Help:Barcodes
     CDS             1185..1865
                     /label=BBa_E1010
     stem_loop       1910..1953
                     /label=stem_loop
     misc_feature    1899..1978
                     /label=BBa_B0010
     terminator      1899..1978
                     /label=BBa_B0010
     stem_loop       1994..2013
                     /label=T7 TE
     misc_feature    2020..2020
                     /label=stop
     polyA_site      2014..2027
                     /label=polya
     misc_feature    1987..2027
                     /label=BBa_B0012
     terminator      1987..2027
                     /label=BBa_B0012
     misc_feature    1167..2027
                     /label=BBa_I13507
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatggct tcctccgaag
     1201 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
     1261 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
     1321 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
     1381 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
     1441 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
     1501 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
     1561 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
     1621 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
     1681 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
     1741 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
     1801 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
     1861 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
     1921 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1981 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//