LOCUS       BBa_I13551              2049 bp    DNA     linear       13-May-2024
DEFINITION  Screening Plamid test component
ACCESSION   BBa_I13551
VERSION     BBa_I13551.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1070..1090
                     /label=RNAse E site
     misc_feature    1078..1079
                     /label=RNAse E cut site (between the bases)
     misc_feature    1091..1124
                     /label=Insertion site
     misc_feature    1125..1145
                     /label=RNAse E site
     misc_feature    1133..1134
                     /label=RNAse E cut site (between the bases)
     stem_loop       1146..1180
                     /label=Hairpin
     misc_feature    1070..1180
                     /label=BBa_I13457
     misc_feature    1193..1196
                     /label=conserved
     RBS             1189..1200
                     /label=BBa_B0034
     CDS             1207..1881
                     /label=mrfp1
     misc_feature    1888..1912
                     /label=Help:Barcodes
     CDS             1207..1887
                     /label=BBa_E1010
     stem_loop       1932..1975
                     /label=stem_loop
     misc_feature    1921..2000
                     /label=BBa_B0010
     terminator      1921..2000
                     /label=BBa_B0010
     stem_loop       2016..2035
                     /label=T7 TE
     misc_feature    2042..2042
                     /label=stop
     polyA_site      2036..2049
                     /label=polya
     misc_feature    2009..2049
                     /label=BBa_B0012
     terminator      2009..2049
                     /label=BBa_B0012
     misc_feature    1189..2049
                     /label=BBa_I13507
     misc_feature    1070..2049
                     /label=BBa_I13528
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt attatttgta
     1081 ttgatctggt gaattaggtc ttcgaatccg atccattgca gtggtattat ttgtattgat
     1141 ctccttacta tctctcgaga gattagtacc tttggagatc tactagagaa agaggagaaa
     1201 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
     1261 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
     1321 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
     1381 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
     1441 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     1501 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     1561 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     1621 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     1681 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     1741 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     1801 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     1861 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
     1921 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1981 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     2041 gcgtttata
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