LOCUS       BBa_I13552              2146 bp    DNA     linear       11-May-2024
DEFINITION  Screening Plamid test component
ACCESSION   BBa_I13552
VERSION     BBa_I13552.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1167..1187
                     /label=RNAse E site
     misc_feature    1175..1176
                     /label=RNAse E cut site (between the bases)
     misc_feature    1188..1221
                     /label=Insertion site
     misc_feature    1222..1242
                     /label=RNAse E site
     misc_feature    1230..1231
                     /label=RNAse E cut site (between the bases)
     stem_loop       1243..1277
                     /label=Hairpin
     misc_feature    1167..1277
                     /label=BBa_I13457
     misc_feature    1290..1293
                     /label=conserved
     RBS             1286..1297
                     /label=BBa_B0034
     CDS             1304..1978
                     /label=mrfp1
     misc_feature    1985..2009
                     /label=Help:Barcodes
     CDS             1304..1984
                     /label=BBa_E1010
     stem_loop       2029..2072
                     /label=stem_loop
     misc_feature    2018..2097
                     /label=BBa_B0010
     terminator      2018..2097
                     /label=BBa_B0010
     stem_loop       2113..2132
                     /label=T7 TE
     misc_feature    2139..2139
                     /label=stop
     polyA_site      2133..2146
                     /label=polya
     misc_feature    2106..2146
                     /label=BBa_B0012
     terminator      2106..2146
                     /label=BBa_B0012
     misc_feature    1286..2146
                     /label=BBa_I13507
     misc_feature    1167..2146
                     /label=BBa_I13528
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagtatt atttgtattg atctggtgaa ttaggtcttc
     1201 gaatccgatc cattgcagtg gtattatttg tattgatctc cttactatct ctcgagagat
     1261 tagtaccttt ggagatctac tagagaaaga ggagaaatac tagatggctt cctccgaaga
     1321 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
     1381 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
     1441 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
     1501 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
     1561 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
     1621 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
     1681 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
     1741 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
     1801 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
     1861 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
     1921 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
     1981 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
     2041 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     2101 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//