LOCUS       BBa_I13553              1811 bp    DNA     linear       15-Jun-2024
DEFINITION  RFP/GFP reporter
ACCESSION   BBa_I13553
VERSION     BBa_I13553.1  
FEATURES             Location/Qualifiers
     misc_feature    1..77
                     /label=PenI
     promoter        23..28
                     /label=-35
     promoter        46..51
                     /label=-10
     misc_feature    31..53
                     /label=dimer left half
     misc_feature    55..76
                     /label=dimer right half
     promoter        1..77
                     /label=BBa_R0074
     misc_feature    90..93
                     /label=conserved
     RBS             86..97
                     /label=BBa_B0034
     CDS             104..823
                     /label=GFP protein
     CDS             104..823
                     /label=BBa_E0040
     misc_feature    86..823
                     /label=BBa_I13500
     misc_feature    832..852
                     /label=RNAse E site
     misc_feature    840..841
                     /label=RNAse E cut site (between the bases)
     misc_feature    853..886
                     /label=Insertion site
     misc_feature    887..907
                     /label=RNAse E site
     misc_feature    895..896
                     /label=RNAse E cut site (between the bases)
     stem_loop       908..942
                     /label=Hairpin
     misc_feature    832..942
                     /label=BBa_I13457
     misc_feature    955..958
                     /label=conserved
     RBS             951..962
                     /label=BBa_B0034
     CDS             969..1643
                     /label=mrfp1
     misc_feature    1650..1674
                     /label=Help:Barcodes
     CDS             969..1649
                     /label=BBa_E1010
     stem_loop       1694..1737
                     /label=stem_loop
     misc_feature    1683..1762
                     /label=BBa_B0010
     terminator      1683..1762
                     /label=BBa_B0010
     stem_loop       1778..1797
                     /label=T7 TE
     misc_feature    1804..1804
                     /label=stop
     polyA_site      1798..1811
                     /label=polya
     misc_feature    1771..1811
                     /label=BBa_B0012
     terminator      1771..1811
                     /label=BBa_B0012
     misc_feature    951..1811
                     /label=BBa_I13507
     misc_feature    832..1811
                     /label=BBa_I13528
     misc_feature    86..1811
                     /label=BBa_I13545
ORIGIN
        1 tcatttccaa ccgaaaaaag gcttgcattt aaatcttaca tatgtaatac tttcaaagac
       61 tacatttgta agatttgtac tagagaaaga ggagaaatac tagatgcgta aaggagaaga
      121 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      181 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      241 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
      301 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
      361 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      421 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      481 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
      541 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
      601 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
      661 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
      721 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
      781 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gtattatttg
      841 tattgatctg gtgaattagg tcttcgaatc cgatccattg cagtggtatt atttgtattg
      901 atctccttac tatctctcga gagattagta cctttggaga tctactagag aaagaggaga
      961 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
     1021 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     1081 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     1141 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     1201 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     1261 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     1321 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     1381 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
     1441 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     1501 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     1561 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
     1621 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
     1681 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1741 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1801 ctgcgtttat a
//