##gff-version 3
##sequence-region BBa_I13553 1 1811
BBa_I13553	.	engineered_region	86	1811	.	+	0	ID=BBa_I13545;Name=BBa_I13545
BBa_I13553	.	engineered_region	832	1811	.	+	0	ID=BBa_I13528;Name=BBa_I13528;Parent=BBa_I13545
BBa_I13553	.	sequence_feature	832	942	.	+	0	ID=BBa_I13457;Name=BBa_I13457;Parent=BBa_I13528
BBa_I13553	.	sequence_feature	840	841	.	+	0	ID=annotation1588511;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13553	.	sequence_feature	853	886	.	+	0	ID=annotation1588512;Name=Insertion site;Parent=BBa_I13457
BBa_I13553	.	stem_loop	908	942	.	+	0	ID=annotation1588515;Name=Hairpin;Parent=BBa_I13457
BBa_I13553	.	sequence_feature	832	852	.	+	0	ID=annotation1588510;Name=RNAse E site;Parent=BBa_I13457
BBa_I13553	.	sequence_feature	887	907	.	+	0	ID=annotation1588513;Name=RNAse E site;Parent=BBa_I13457
BBa_I13553	.	sequence_feature	895	896	.	+	0	ID=annotation1588514;Name=RNAse E cut site (between the bases);Parent=BBa_I13457
BBa_I13553	.	engineered_region	951	1811	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_I13528
BBa_I13553	.	CDS	969	1649	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_I13553	.	CDS	969	1643	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I13553	.	engineered_tag	1650	1674	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I13553	.	terminator	1771	1811	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_I13553	.	polyA_site	1798	1811	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I13553	.	stop_codon	1804	1804	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I13553	.	engineered_region	1771	1811	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I13553	.	stem_loop	1778	1797	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I13553	.	ribosome_entry_site	951	962	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_I13553	.	conserved_region	955	958	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13553	.	terminator	1683	1762	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_I13553	.	engineered_region	1683	1762	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I13553	.	stem_loop	1694	1737	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I13553	.	engineered_region	86	823	.	+	0	ID=BBa_I13500;Name=BBa_I13500;Parent=BBa_I13545
BBa_I13553	.	CDS	104	823	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13500
BBa_I13553	.	CDS	104	823	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I13553	.	ribosome_entry_site	86	97	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13500
BBa_I13553	.	conserved_region	90	93	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I13553	.	promoter	1	77	.	+	0	ID=BBa_R0074;Name=BBa_R0074
BBa_I13553	.	non_covalent_binding_site	31	53	.	+	0	ID=annotation319788;Name=dimer left half;Parent=BBa_R0074
BBa_I13553	.	non_covalent_binding_site	55	76	.	+	0	ID=annotation319789;Name=dimer right half;Parent=BBa_R0074
BBa_I13553	.	promoter	46	51	.	+	0	ID=annotation319212;Name=-10;Parent=BBa_R0074
BBa_I13553	.	operator	1	77	.	+	0	ID=annotation302713;Name=PenI;Parent=BBa_R0074
BBa_I13553	.	promoter	23	28	.	+	0	ID=annotation319211;Name=-35;Parent=BBa_R0074
>BBa_I13553
tcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttgta
ctagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag
atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaa
atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgcttt
gcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaacta
tatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgta
tacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttc
aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccac
acaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatt
acacatggcatggatgaactatacaaataataatactagagtattatttgtattgatctggtgaattaggtcttcgaat
ccgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattagtacctttggagatctactag
agaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaagg
ttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactg
aaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacg
ttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaactt
cgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgt
ggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacc
cggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaa
aaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccac
aacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagt
gctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata